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Identification of microRNA transcriptome reveals that miR-100 is involved in the renewal of porcine intestinal epithelial cells

  • Lijun Zou
  • Xia XiongEmail author
  • Huansheng Yang
  • Kexing Wang
  • Jian Zhou
  • Dinghong Lv
  • Yulong YinEmail author
Research Paper

Abstract

MicroRNAs play important roles in various cellular processes, including differentiation, proliferation and survival. Using a pig model, this study sought to identify the miRNAs responsible for crypt-villus axis renewal of the small intestinal epithelium. Compared to the villus upper cells, there were 15 up-regulated and 41 down-regulated miRNAs in the crypt cells of the jejunum. Notably, we found that miR-100 was expressed more in the villus upper cells than in the crypt cells, suggesting an effect on intestinal epithelium differentiation. Overexpression of miR-100 increased the activity of alkaline phosphatase, confirming that miR-100 promoted IPEC-J2 cell differentiation. MiR-100 can inhibit cell proliferation as evidenced by CCK-8 and cell cycle assay results. We also showed that miR-100 significantly inhibited the migration of IPEC-J2 cells and promoted cell apoptosis through caspase-3-dependent cleavage of Bcl-2. Furthermore, FGFR3 was identified as a potential target of miR-100 by bioinformatics analysis. We confirmed that overexpression of miR-100 suppressed FGFR3 expression in IPEC-J2 cells by directly targeting the FGFR3 3′-UTR. This is the first report of miRNAs acting on the renewal of the intestinal crypt-villus axis. Our results also showed that miR-100 promotes the differentiation and apoptosis, and inhibits the proliferation and migration of enterocytes of pigs.

Keywords

MicroRNAs suckling piglets crypt-villus axis cell renewal IPEC-J2 cells 

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Notes

Acknowledgements

This work was supported by the Key Programs of Frontier Scientific Research of the Chinese Academy of Sciences (QYZDYSSW- SMC008), National Program on Key Basic Research Project (2016YFD0500504), National Nature Science Foundation of China (31330075; 31572420), Hunan Provincial Natural Science Foundation of China (2018JJ3094; 2018JJ1028), Research Foundation of Education Bureau of Hunan Province, China (18B476) and Hunan Provincial Innovation Foundation for Postgraduate (CX2016B172).

Supplementary material

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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, School of Life SciencesHunan Normal UniversityChangshaChina
  2. 2.Key Laboratory for Agro-Ecological Processes in Subtropical Regions, Institute of Subtropical Agriculturethe Chinese Academy of SciencesChangshaChina
  3. 3.Laboratory of Basic BiologyHunan First Normal UniversityChangshaChina

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