Science China Life Sciences

, Volume 61, Issue 7, pp 826–835 | Cite as

Differential expression of FOXO1 during development and myoblast differentiation of Qinchuan cattle and its association analysis with growth traits

  • Yujia Sun
  • Kunpeng Liu
  • Yongzhen Huang
  • Xianyong Lan
  • Hong ChenEmail author
Research Paper


Our previous work reported a relationship between FOXO1 mutations and growth of Qinchuan (QC) cattle. Here, we performed differential expression analysis of FOXO1 and its association analysis with growth traits in QC cattle. First, we measured the expression of the FOXO1 gene in nine tissues during three developmental stages. The results showed that FOXO1 was abundantly expressed in tissues of calves but was strongly repressed in adulthood, although there was significant transcription in skeletal muscle. FOXO1 expression showed gradual up-regulation during differentiation of primary bovine skeletal muscle cells. We also identified six SNPs of the bovine FOXO1 gene by sequencing DNA pools of samples from 488 individuals, and association analysis indicated that five SNPs were significantly associated with some growth traits in the QC population. We further analyzed four haplotype combinations of the six SNPs and found significant correlation with body length (P<0.01). In conclusion, FOXO1 participates in bovine myocyte differentiation and expression, and may be a strong candidate as a gene that affects growth traits that could be exploited in a QC cattle breeding program. More generally, our data provide a new theoretical basis for QC beef breeding and beef quality improvement.


tissues expression myoblast differentiation sequence variants association analysis Qinchuan cattle 


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This work was supported by the National Natural Science Foundation of China (31272408), Agricultural Science and Technology Innovation Projects of Shaanxi Province (2012NKC01-13), Program of National Beef Cattle Industrial Technology System (CARS-38) and National High Technology Research and Development Program of China (2013AA102505).

Supplementary material

11427_2017_9205_MOESM1_ESM.docx (17 kb)
Table S1 Primers information for PCR amplification of bovine FOXO1 gene


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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yujia Sun
    • 1
  • Kunpeng Liu
    • 1
  • Yongzhen Huang
    • 1
  • Xianyong Lan
    • 1
  • Hong Chen
    • 1
    • 2
    Email author
  1. 1.College of Animal Science and TechnologyNorthwest A&F University, Shaanxi Key Laboratory of Molecular Biology for AgricultureYanglingChina
  2. 2.Institute of Cellular and Molecular BiologyJiangsu Normal UniversityXuzhouChina

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