Genome sequencing and phylogenetic analysis of Banna virus (genus Seadornavirus, family Reoviridae) isolated from Culicoides
- 78 Downloads
In an investigation of blood-sucking insects and arboviruses, a virus (YN12243) was isolated from Culicoides samples collected in the Sino-Burmese border region of Yunnan Province, China. The virus caused cytopathic effect (CPE) in C6/36 cells and passaged stably. Polyacrylamide gel analysis showed that the genome of YN12243 was composed of 12 segments of double-stranded RNA (dsRNA), with a distribution pattern of 6-6. The nucleotide and amino acid sequences of the coding region (1‒12 segments) were 17,803 bp and 5,925 amino acids in length, respectively. The phylogenetic analysis of VP1 protein (RdRp) revealed that YN12243 belonged to genus Seadornavirus of family Reoviridae, and further analysis indicated that YN12243 belongs to the Banna virus (BAV) genotype A2. Additionally, YN12243 was located in the same evolutionary cluster as BAV strains isolated from different mosquito species, suggesting that the BAV isolated from Culicoides does not have species barriers. These results indicate that Culicoides can also be a vector for BAV. In view of the hematophagous habits of Culicoides on cattle, horses, deer, and other large animals, as well as the possibility of spreading and causing a variety of animal arboviral diseases, it is important to improve infection detection and monitor the BAV in large livestock.
KeywordsBanna virus Seadornavirus Reoviridae mosquito Culicoides
Unable to display preview. Download preview PDF.
This work was supported by the National Natural Science Foundation of China (81290342, 81501757), the Special National Project on Research and Development of Key Biosafety Technologies (2016YFC1201904), the National Key Plan for Scientific Research and Development of China (2016YFD0500300) and the development grants of State Key Laboratory of Infectious Disease Prevention and Control (2014 SKLID103, 2015SKLID505).
- Attoui, H., Billoir, F., Biagini, P., de Micco, P., and de Lamballerie, X. (2000). Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J Gen Virol 81, 1507–1515.CrossRefPubMedGoogle Scholar
- Attoui, H., Mohd Jaafar, F., de Lamballerie, X., and Mertens, P.P. (2005b). Seadornavirus, Reoviridae. In: Eighth Report of the International Committee on Taxonomy of Viruses, C.M., Fauquet, M.A., Mayo, J., Maniloff, U., Desselberger, and L.A., Ball, eds. (London: Elsevier Academic Press), pp. 504–510.Google Scholar
- Kim, H.K., Yoon, S.W., Kim, D.J., Koo, B.S., Noh, J.Y., Kim, J.H., Choi, Y.G., Na, W., Chang, K.T., Song, D., and Jeong, D.G. (2016). Detection of severe acute respiratory syndrome-like, middle east respiratory syndrome-like bat coronaviruses and group H rotavirus in faeces of Korean Bats. Transbound Emerg Dis 63, 365–372.CrossRefPubMedGoogle Scholar
- Deng, Y.Q., Zhao, H., Li, X.F., Zhang, N.N., Liu, Z.Y., Jiang, T., Gu, D.Y., Shi, L., He, J.A., Wang, H.J., Sun, Z.Z., Ye, Q., Xie, D.Y., Cao, W.C., and Qin, C.F. (2016). Isolation, identification and genomic characterization of the Asian lineage Zika virus imported to China. Sci China Life Sci 59, 428–430.CrossRefPubMedGoogle Scholar
- Kou, M., Zhu, J., Yang, H., Xiao, L., Wang, J., Miao, H., Wang, J., and Li, H. (2016). First isolation and idengtification of Banna virus from Culicoides pools in Yunnan (in Chinese). Chin J Exp Clin Virol 30, 1–4.Google Scholar
- Liu, H., and Liang, G. (2010a). Banna virus and its infection (in Chinese). Chin J Vector Biol Control 21, 500–502.Google Scholar
- Liu, H., and Liang, G.D. (2016a). The genotype A specific primers for amplifying and sequencing the whole genome of Banna virus (in Chinese). Chin J Vector Biol Control 27, 533–538.Google Scholar
- Nabeshima, T., Thi Nga, P., Guillermo, P., Parquet, M.C., Yu, F., Thanh Thuy, N., Minh Trang, B., Tran Hien, N., Sinh Nam, V., Inoue, S., Hasebe, F., and Morita, K. (2008). Isolation and molecular characterization of Banna virus from mosquitoes, Vietnam. Emerg Infect Dis 14, 1276–1279.CrossRefPubMedPubMedCentralGoogle Scholar
- Tao, S.J., and Chen, B.Q. (2005). Studies of Coltivirus in China. Chin Med J (Engl) 118, 581–586.Google Scholar
- Wang, J., Li, H., He, Y., Zhou, Y., Meng, J., Zhu, W., Chen, H., Liao, D., and Man, Y. (2015). Isolation and genetic characterization of Mangshi virus: a newly discovered Seadornavirus of the Reoviridae family found in Yunnan Province, China. PLoS ONE 10, e0143601.CrossRefPubMedPubMedCentralGoogle Scholar
- Xu, P., Wang, Y., Zuo, J., Lin, J., and Xu, P. (1990a). New orbiviruses isolated from patients with unknown fever and encephalitis in Yunnan province (in Chinese). Chin J Virol 6, 27–33.Google Scholar
- Xu, P., Wang, Y., Zuo, J., Che, Y., Peng, H., Huang, Z., Tang, G., and Xu, P. (1990b). Recovery of the same type of virus as human new orbivirus (in Chinese). Chin J Virol 6, 328–332.Google Scholar
- Yang, J.M., Yin, G.Q., Feng, Y.H., Luo, Z.Y., and Jiao, J.F. (1996). Co-infection of Coltivirus and Japanese encephalitis virus (in Chinese). J Nanjing Railway Med College 15, 169.Google Scholar