Science China Life Sciences

, Volume 57, Issue 3, pp 327–335 | Cite as

Ingestion of Lactobacillus strain reduces anxiety and improves cognitive function in the hyperammonemia rat

  • Jia Luo
  • Tao Wang
  • Shan Liang
  • Xu Hu
  • Wei Li
  • Feng JinEmail author
Open Access
Research Paper


Evidence suggests that the hyperammonemia (HA)-induced neuroinflammation and alterations in the serotonin (5-HT) system may contribute to cognitive decline and anxiety disorder during hepatic encephalopathy (HE). Probiotics that maintain immune system homeostasis and regulate the 5-HT system may be potential treatment for HA-mediated neurological disorders in HE. In this study, we tested the efficacy of probiotic Lactobacillus helveticus strain NS8 in preventing cognitive decline and anxiety-like behavior in HA rats. Chronic HA was induced by intraperitoneal injection of ammonium acetate for four weeks in male Sprague-Dawley rats. HA rats were then given Lactobacillus helveticus strain NS8 (109 CFU mL−1) in drinking water as a daily supplementation. The Morris water maze task assessed cognitive function, and the elevated plus maze test evaluated anxiety-like behavior. Neuroinflammation was assessed by measuring the inflammatory markers: inducible nitric oxide synthase, prostaglandin E2, and interleukin-1 β in the brain. 5-HT system activity was evaluated by measuring 5-HT and its metabolite, 5-HIAA, and the 5-HT precursor, tryptophan. Probiotic treatment of HA rats significantly reduced the level of inflammatory markers, decreased 5-HT metabolism, restored cognitive function and improved anxiety-like behavior. These results indicate that probiotic L. helveticus strain NS8 is beneficial for the treatment of cognitive decline and anxiety-like behavior in HA rats.


hyperammonemia probiotics cognition anxiety neuroinflammation serotonin 


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Copyright information

© The Author(s) 2014

Authors and Affiliations

  • Jia Luo
    • 1
    • 2
  • Tao Wang
    • 1
  • Shan Liang
    • 1
    • 2
  • Xu Hu
    • 1
  • Wei Li
    • 1
    • 2
  • Feng Jin
    • 1
    Email author
  1. 1.Key Laboratory of Mental Health, Institute of PsychologyChinese Academy of SciencesBeijingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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