Science China Life Sciences

, Volume 57, Issue 3, pp 336–339 | Cite as

Urimem, a membrane that can store urinary proteins simply and economically, makes the large-scale storage of clinical samples possible

Open Access
Research Paper

Abstract

By nature, biomarker is the measurable change associated with a physiological or pathophysiological process. Unlike blood which has mechanisms to minimize changes and to keep the internal environment homeostatic, urine is more likely to reflect changes of the body and is a better biomarker source. Because of its potential in biomarker discovery, urinary proteins should be preserved comprehensively as the duration of the patients’ corresponding medical records. Here, we propose a method to adsorb urinary proteins onto a membrane we named Urimem. This simple and inexpensive method requires minimal sample handling, uses no organic solvents, and is environmentally friendly. Urine samples were filtered through the membrane, and urinary proteins were adsorbed onto the membrane. The proteins on the membrane were dried and stored in a vacuum bag, which keeps the protein pattern faithfully preserved. The membrane may even permit storage at room temperature for weeks. Using this simple and inexpensive method, it is possible to begin preserving urine samples from all consenting people. Thus, medical research especially biomarker research can be conducted more economically. Even more objective large-scale prospective studies will be possible. This method has the potential to change the landscape of medical research and medical practice.

Keywords

urine proteins biological-sample preservation PVDF/NC membrane 

References

  1. 1.
    Gao YH. Urine-an untapped goldmine for biomarker discovery? Sci China Life Sci, 2013, 56: 1145–1146PubMedCrossRefGoogle Scholar
  2. 2.
    Szewczyk B, Summers DF. Preparative elution of proteins blotted to Immobilon membranes. Anal Biochem, 1988, 168: 48–53PubMedCrossRefGoogle Scholar
  3. 3.
  4. 4.
    Kuno H, Kihara HK. Simple microassay of protein with membrane filter. Nature, 1967, 215: 974–975PubMedCrossRefGoogle Scholar
  5. 5.
    Kihara HK, Kuno H. Microassay of protein with nitrocellulose membrane filters. Anal Biochem, 1968, 24: 96–105PubMedCrossRefGoogle Scholar
  6. 6.
    Nakamura K, Tanaka T, Kuwahara A, Takeo K. Microassay for proteins on nitrocellulose filter using protein dye-staining procedure. Anal Biochem, 1985, 148: 311–319PubMedCrossRefGoogle Scholar
  7. 7.
    Anderson PJ. The recovery of nitrocellulose-bound protein. Anal Biochem, 1985, 148: 105–110PubMedCrossRefGoogle Scholar

Copyright information

© The Author(s) 2013

Authors and Affiliations

  1. 1.Department of Physiology and Pathophysiology, National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences/Peking Union Medical CollegeBeijingChina
  2. 2.Department of Nephrology, Peking Union Medical College HospitalChinese Academy of Medical SciencesBeijingChina

Personalised recommendations