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Science China Life Sciences

, Volume 56, Issue 7, pp 619–627 | Cite as

Mining of a streptothricin gene cluster from Streptomyces sp. TP-A0356 genome via heterologous expression

  • JinE Li
  • ZhengYan Guo
  • Wei Huang
  • XiangXi Meng
  • GuoMin Ai
  • GongLi Tang
  • YiHua Chen
Open Access
Research Paper Special Topic: Biosynthesis and Regulation of Secondary Metabolites in Microorganisms

Abstract

Streptothricins (STs) are used commercially to treat bacterial and fungal diseases in agriculture. Mining of the sequenced microbial genomes uncovered two cryptic ST clusters from Streptomyces sp. C and Streptomyces sp. TP-A0356. The ST cluster from S. sp. TP-A0356 was verified by successful heterologous expression in Streptomyces coelicolor M145. Two new ST analogs were produced together with streptothricin F and streptothricin D in the heterologous host. The ST cluster was further confirmed by inactivation of gene stnO, which was proposed encoding an aminomutase supplying β-lysines for the poly-β-Lys chain formation. A putative biosynthetic pathway for STs is proposed based on bioinformatics analyses of the ST genes and experimental evidence.

Keywords

genome mining streptothricin Streptomyces heterologous expression biosynthesis 

Supplementary material

11427_2013_4504_MOESM1_ESM.pdf (868 kb)
Supplementary material, approximately 867 KB.

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Copyright information

© The Author(s) 2013

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Resources, Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  2. 2.State Key Laboratory of Bio-organic and Natural Product Chemistry, Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghaiChina
  3. 3.University of Chinese Academy of SciencesBeijingChina

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