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Science China Life Sciences

, Volume 56, Issue 4, pp 293–297 | Cite as

A novel strategy to inhibit the reproduction and translation of hepatitis C virus

  • AiPing Duan
  • LiMin Ning
  • Chao Li
  • YaFei Hou
  • NaNa Yang
  • LiZhou Sun
  • GenXi LiEmail author
Open Access
Cover Article

Abstract

Hepatitis C virus (HCV), a positive single-stranded RNA virus, is a major cause of liver disease in humans. Herein we report a novel strategy to inhibit the reproduction and translation of HCV using a short RNA, named an Additional RNA, to activate the endonuclease activity of Argonaute 2 (Ago2). In the presence of the Additional RNA, the HCV genome RNA has the requisite 12 nucleotides of base-pairing with microRNA-122. This activates the endonuclease activity of Ago2, resulting in cleavage and release of the HCV genome RNA from Ago2 and microRNA-122. The free HCV genome RNA would be susceptible to intracellular degradation, effectively inhibiting its reproduction and translation. This study presents a new method to inhibit HCV that may hold great potential for HCV treatment in the future.

Keywords

hepatitis C virus reproduction and translation Argonaute 2 protein electro-analysis biosensor 

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Copyright information

© The Author(s) 2013

Authors and Affiliations

  • AiPing Duan
    • 1
  • LiMin Ning
    • 1
  • Chao Li
    • 1
  • YaFei Hou
    • 2
  • NaNa Yang
    • 3
  • LiZhou Sun
    • 3
  • GenXi Li
    • 1
    • 2
    Email author
  1. 1.Department of Biochemistry and State Key Laboratory of Pharmaceutical BiotechnologyNanjing UniversityNanjingChina
  2. 2.Laboratory of Biosensing Technology, School of Life SciencesShanghai UniversityShanghaiChina
  3. 3.Department of Obstetricsthe First Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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