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Science China Life Sciences

, Volume 56, Issue 1, pp 90–100 | Cite as

Four new species of the genus Hymenoscyphus (fungi) based on morphology and molecular data

  • HuanDi Zheng
  • WenYing Zhuang
Open Access
Research Paper

Abstract

Four new species of Hymenoscyphus (H. brevicellulus, H. hyaloexcipulus, H. microcaudatus, and H. subsymmetricus) and a new Chinese record (H. subpallescens) are described. These five species share common characteristics: small apothecia (<3 mm in diameter); hymenium whitish, pale yellow, to yellow in color; ectal excipulum of textura prismatica; asci arising from simple septa; ascospores scutuloid and guttulate; saprophytic nutrition; and leaf habitats, except for H. subsymmetricus, which grows on herbaceous stems. Phylogenetic analyses of internal transcribed spacer nuclear ribosomal DNA sequences, the universal DNA barcode for fungi, for 16 species in the genus indicated that these taxa were closely related to H. microserotinus, in accordance with their morphological features, but represented independent species. The distinguishing features of each new species from its relatives are discussed, and their phylogenetic relationships explored.

Keywords

Helotiaceae morphology sequence analyses taxonomy 

References

  1. 1.
    Kirk P, Cannon P, Minter D, et al., eds. Dictionary of the Fungi. 10th ed. Wallingford: CABI, 2008Google Scholar
  2. 2.
    Gray S F, ed. A Natural Arrangement of British Plants. Vol. 1. London: C. Baldwin Printer, 1821Google Scholar
  3. 3.
    Dennis R W G. Remarks on the genus Hymenoscyphus S.F. Gray, with observation on sundry species referred by Saccardo and others to the genera Helotium, Pezizella or Phialea. Persoonia, 1964, 3: 29–80Google Scholar
  4. 4.
    Phillips W A, eds. A Manual of the British Discomycetes with Descriptions of All the Species of Fungi Hitherto Found in Britain, Included in the Family, and Illustrations of the Genera. London: Kegan Paul, Trench & Co., 1887Google Scholar
  5. 5.
    Dennis R W G. A revision of the British Helotiaceae in the Herbarium of the Royal Botanic Garden, Kew, with notes on related European species. Mycol Pap, 1956, 62: 1–216Google Scholar
  6. 6.
    Lizoň P. The genus Hymenoscyphus (Helotiales) in Slovakia, Czechoslovakia. Mycotaxon, 1992, 45: 1–59Google Scholar
  7. 7.
    White W L. Studies in the genus Helotium, III. History and diagnosis of certain European and North American foliicolous species. Farlowia, 1943, 1: 135–170Google Scholar
  8. 8.
    Dumont K P. Leotiaceae II. A preliminary survey of the neotropical species referred to Helotium and Hymenoscyphus. Mycotaxon, 1981, 12: 313–371Google Scholar
  9. 9.
    Dumont K P. Leotiaceae III. Notes on selected temperate species referred to Helotium and Hymenoscyphus. Mycotaxon, 1981, 13: 59–84Google Scholar
  10. 10.
    Dumont K P, Carpenter S E. Los Hongos de Colombia—VII: Leotiaceae—IV: Hymenoscyphus caudatus and related species from Colombia and adjacent regions. Caldasia, 1982, 13: 567–602Google Scholar
  11. 11.
    Thind K S, Sharma M P. The genus Hymenoscyphus S. F. Gray in Indian. Nova Hedwigia, 1980, 32: 121–132Google Scholar
  12. 12.
    Sharma M P. Diversity in the Himalayan Hymenoscyphus S. F. Gray: an overview. In: Khullar S P and Sharma M P, eds. Himalayan Botanical Researches. New Delhi: Ashish Publishing House, 1991. 107–211Google Scholar
  13. 13.
    Zhang Y H, Zhuang W Y. Re-examinations of Helotium and Hymenoscyphus (Helotiales, Helotiaceae): specimens on deposit in HMAS. Mycotaxon, 2002, 81: 35–43Google Scholar
  14. 14.
    Zhang Y H, Zhuang W Y. New species and new Chinese records of Hymenoscyphus (Helotiales). Mycosystema, 2002, 21: 493–496Google Scholar
  15. 15.
    Zhang Y H. Taxonomy of Hymenoscyphus (Helotiales) in China and a phylogenetic approach to the genus (in Chinese). Dissertation for Doctoral Degree. Beijing: Institute of Microbiology, Chinese Academy of Sciences, 2002Google Scholar
  16. 16.
    Abdel-Raheem A, Shearer C A. Extracellular enzyme production by freshwater ascomycetes. Fungal Divers, 2002, 11: 1–19Google Scholar
  17. 17.
    Thines E, Anke H, Steglich W, et al. New botrydial sesquiterpenoids from Hymenoscyphus epiphyllus. Z Naturforsch C, 1997, 52: 413–420PubMedGoogle Scholar
  18. 18.
    Queloz V, Grünig C R, Berndt R, et al. Cryptic speciation in Hymenoscyphus albidus. Forest Pathol, 2010, 40: 1–14CrossRefGoogle Scholar
  19. 19.
  20. 20.
    Baral H O, Galán R, López J, et al. Hymenoscyphus crataegi (Helotiales), a new species from Spain and its phylogenetic position within the genus Hymenoscyphus. Sydowia, 2006, 58: 145–162Google Scholar
  21. 21.
    Han J G, Shin H D. Hymenoscyphus ginkgonis sp. nov. growing on leaves of Ginkgo biloba. Mycotaxon, 2008, 103: 189–195Google Scholar
  22. 22.
    Chandelier A, André F, Laurent F. Detection of Chalara fraxinea in common ash (Fraxinus excelsior) using real time PCR. Forest Pathol, 2010, 40: 87–95CrossRefGoogle Scholar
  23. 23.
    Bengtsson S B K, Vasaitis R, Kirisits T, et al. Population structure of Hymenoscyphus pseudoalbidus and its genetic relationship to Hymenoscyphus albidus. Fungal Ecol, 2012, 5: 147–153CrossRefGoogle Scholar
  24. 24.
    Kraj W, Zarek M, Kowalski T. Genetic variability of Chalara fraxinea, dieback cause of European ash (Fraxinus excelsior L.). Mycol Prog, 2012, 11: 37–45CrossRefGoogle Scholar
  25. 25.
    Teng S C. Note on discomycetes from China. Sinensia, 1934, 5: 431–465Google Scholar
  26. 26.
    Zhuang W Y, Korf R P. Some new species and new records of discomycetes in China. III. Mycotaxon, 1989, 35: 297–312Google Scholar
  27. 27.
    Zhuang W Y. Some new species and new records of Discomycetes in China. V. Mycotaxon, 1995, 56: 31–40Google Scholar
  28. 28.
    Zhuang W Y, Wang Z. Some new species and new records of discomycetes in China VIII. Mycotaxon, 1998, 66: 429–438Google Scholar
  29. 29.
    Zhuang W Y. Some new species and new records of Discomycetes in China. XI. Mycotaxon, 2003, 87: 467–473Google Scholar
  30. 30.
    Zhang Y H, Zhuang W Y. Phylogenetic relationships of some members in the genus Hymenoscyphus (Ascomycetes, Helotiales). Nova Hedwigia, 2004, 78: 475–484CrossRefGoogle Scholar
  31. 31.
    Zheng H D, Zhuang W Y. Notes on the genus Hymenoscyphus from Tropical China. J Fungal Res, 2011, 9: 212–215Google Scholar
  32. 32.
    Schoch C L, Seifert K A, Huhndorf S, et al. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA, 2012, 109: 6241–6246PubMedPubMedCentralCrossRefGoogle Scholar
  33. 33.
    White T, Bruns T D, Lee A, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M A, Gelfand D H, Snisky J J, et al., eds. PCR Protocols. London: Academic Press, 1990. 315–322Google Scholar
  34. 34.
    Hall T A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser, 1999, 41: 95–98Google Scholar
  35. 35.
    Thompson J D, Gibson T J, Plewniak F, et al. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25: 4876–4882PubMedPubMedCentralCrossRefGoogle Scholar
  36. 36.
    Swofford D L. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4b10. Sinauer Associates, Sunderland, Massachusetts. 2003Google Scholar
  37. 37.
    Page R D M. TREEVIEW: An application to display phylogenetic trees on personal computers. Comp Appl Biosci, 1996, 12: 357–358PubMedGoogle Scholar
  38. 38.
    MegAlign (DNASTAR, Lasergene): Software for DNA and Protein Sequence Alignments and Analysis. Version 7.1.0. 1993–2006Google Scholar
  39. 39.
    Dennis R W G. New or interesting British microfungi III. Kew Bull, 1975, 30: 345–365CrossRefGoogle Scholar
  40. 40.
    Hawksworth D L. The fungal dimension of biodiversity: magnitude, significance, and conservation. Mycol Res, 1991, 95: 641–655CrossRefGoogle Scholar
  41. 41.
    Hawksworth D L. The magnitude of fungal diversity: the 1.5 million species estimate revisited. Mycol Res, 2001, 105: 1422–1432CrossRefGoogle Scholar
  42. 42.
    Taylor J W, Jacobson D J, Kroken S, et al. Phylogenetic species recognition and species concepts in fungi. Fungal Genet Biol, 2000, 31: 21–32PubMedCrossRefGoogle Scholar
  43. 43.
    Giraud T, Refrégier G, Le Gac M, et al. Speciation in fungi. Fungal Genet Biol, 2008, 45: 791–802PubMedCrossRefGoogle Scholar

Copyright information

© The Author(s) 2012

Authors and Affiliations

  1. 1.State Key Laboratory of Mycology, Institute of MicrobiologyChinese Academy of SciencesBeijingChina

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