Generation of fad2 transgenic mice that produce omega-6 fatty acids
- 57 Downloads
Fatty acid desaturase-2 (FAD2) introduces a double bond in position Δ12 in oleic acid (18:1) to form linoleic acid (18:2 n-6) in higher plants and microbes. A new transgenic expression cassette, containing CMV promoter/fad2 cDNA/SV40 polyA, was constructedto produce transgenic mice. Among 63 healthy offspring, 10 founders (15.9%) integrated the cotton fad2 transgene into their genomes, as demonstrated by PCR and Southern blotting analysis. All founder mice were fertile and heterozygous fad2 female and nontransgenic littermates were used for fatty acid analysis using gas chromatography. One fad2 transgenic line showed substantial differences in the fatty acid profiles and the level of linoleic acid was increased 19% (P<0.05) in transgenic muscles compared to their nontransgenic littermates. Moreover, it exhibited an 87% and a 9% increase (P<0.05) in arachidonic acid (20:4 n-6) in muscles and liver, compared to their nontransgenic littermates. The results indicate that the plant fad2 gene can be functionally expressed in transgenic mice and may playan active role in conversion of oleic acid into linoleic acid.
Keywordsfatty acid desaturase FAD2 transgenic mouse oleic acid linoleic acid arachidonic acid
Unable to display preview. Download preview PDF.
- 6.Wang Y, An X R, Liu Q, et al. Establishment of transgenic mice integrated with gene encoding cotton omega-6 fatty acid desaturase-2. J Agri Biotechnol, 2005, 13: 207–211, 1:CAS:528:DC%2BD2MXht1akurrOGoogle Scholar
- 7.Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Mannual 3th ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 2001.Google Scholar
- 21.Almendingen K, Hostmark A T, Fausa O, et al. Familial adenomatous polyposis patients have high levels of arachidonic acid and docosa-hexaenoic acid and low levels of linoleic acid and alpha-linolenic acid in serum phospholipids. Int. J Cancer, 2007, 120: 632–637, 10.1002/ijc.22337, 1:CAS:528:DC%2BD28XhtlGqsrfF, 17096349CrossRefPubMedGoogle Scholar
- 24.Kilian M, Mautsch I, Gregor J I, et al. Influence of conjugated vs. conventional linoleic acid on liver metastasis and hepatic lipidper-oxidation in BOP-induced pancreatic cancer in Syrian hamster. Prostaglandins Leukot. Essent Fatty Acids, 2002, 67: 223–228, 10.1054/plef.2002.0422, 1:CAS:528:DC%2BD38XosVSiu7k%3DCrossRefGoogle Scholar