Science in China Series C: Life Sciences

, Volume 51, Issue 11, pp 1020–1024 | Cite as

Purification and characterization of a novel cholesterol-lowering protein from the seeds of Senna obtusifolia

  • ChuHua LiEmail author
  • Mei Li
  • WenRui Chang
  • BaoJiang Guo


“Juemingzi”, a source of traditional Chinese herbal medicine, has been demonstrated to play a role in decreasing serum cholesterol concentration. In this study, a novel protein, which has shown an inhibitory effect on cholesterol biosynthesis, was isolated from Senna obtusifolia L. seed by gel filtration and ion exchange chromatography. The novel protein’s molecular mass was 19.7 kD and its pI was 4.80. Both SDS-PAGE and isoelectric-focusing (IEF) revealed a single Coomassie brilliant blue stained band, indicating that the novel protein was a single peptide. The N-terminal amino acid sequence of the protein was IPYISASFPLNIEFLPSE, which had no similarity with any other protein sequences in the NCBI protein database. Circular dichroism (CD) signals indicated that S. obtusifolia seed protein contained 12.5% α-helix, 55.6% β-sheet, and 31.9% random coil


Senna obtusifolia L. seed novel protein purification biochemical properties cholesterol biosynthesis 


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  1. 1.
    Chen W X, Diao G J, Jiang W J. Effect of Senna obtusifolia L. seed on model of hypercholesterol in mice. Chin Tradit Herb Drugs (in Chinese), 1991, 22: 72–73, 1:CAS:528:DyaK3MXmvVKntrc%3DGoogle Scholar
  2. 2.
    Patil U K, Saraf S, Dixit V K. Hypolopidemic activity of seeds of Cassia tora Linn. J Ethnopharmacol, 2004, 90: 249–252, 15013188, 10.1016/j.jep.2003.10.007CrossRefPubMedGoogle Scholar
  3. 3.
    Choi J S, Lee H, Yark K. In vitro antimutagenic effects of anthraquinone aglyones and naphthopyrone glycosides from Cassia tora. Plant Med, 1997, 63: 11–14, 10.1055/s-2006-957593, 1:CAS:528:DyaK2sXhtlCntrk%3DCrossRefGoogle Scholar
  4. 4.
    Guo C Y, Horn W C, Pin D D. Extraction and identification of an antioxidative component from Jue Ming Zi (Cassia tora L.). J Agric Food Chem, 1998, 46: 820–824, 10.1021/jf970690zCrossRefGoogle Scholar
  5. 5.
    Li C H, Li X E, Guo B J. China Patent 200510036857.XGoogle Scholar
  6. 6.
    Vadivel V, Janardhanan K. Agrobotanical traits and chemical composition of Cassia obtusifolia L.: A lesser-known legume of the Western Ghats region of South India. Plant Foods Human Nitrit, 2002, 57: 151–164, 10.1023/A:1015218512666, 1:CAS:528:DC%2BD38XkslSkt74%3DCrossRefGoogle Scholar
  7. 7.
    Li C H, Li X E, Guo B J. The effects of cassia seed extracts on reducing blood lipid. J South China Norm Univ (in Chinese), 2002, 4: 29–31Google Scholar
  8. 8.
    Li X E, Guo B J. Effect of protein and anthraquinone glucosides from Senna obtusifolia seed on serum lipid of hyperlipidemia rats. Chin J Chin Mater Med (in Chinese), 2002, 27: 374–376, 1:CAS:528:DC%2BD3sXksl2ltbk%3DGoogle Scholar
  9. 9.
    Shen X C, Liang H, He X W, et al. Recent trends and spectroscopic methods for analysis of the protein conformation with circular dichroism. Chin J Analyt Chem (in Chinese), 2004, 32: 388–394, 1:CAS:528:DC%2BD2cXjsVGls78%3DGoogle Scholar
  10. 10.
    Han F, Gu Z L. Inhibition effect of Baicao Jiangzhiling on cholesterol biosynthesis on cells. J Suzhou Univ (in Chinese), 2002, 22: 517–519Google Scholar
  11. 11.
    Li C H, Fang K Y, Li X E, et al. Effects of Cassia obtusifolia Linn seed anthraquinones on hyperlipidemia and cholesterol biosynthesis in cell. J Clin Rehabilit Tissue Eng Res, 2008, 12(33): 6593–6596Google Scholar
  12. 12.
    Takamatsu K, Tachibana N, Matsunoto I, et al. Soy protein functionality and nutrigenomic analisis. BioFactors, 2004, 21: 49–53, 15630169, 1:CAS:528:DC%2BD2cXhtFGnsr3ICrossRefPubMedGoogle Scholar
  13. 13.
    Anderson J W, Johnstone B M, Cook-Newell M F. Meta-analysis of the effects of soy protein intake on serum lipids. New Engl J Med, 1995, 333: 276–282, 7596371, 10.1056/NEJM199508033330502, 1:CAS:528:DyaK2MXntlKht74%3DCrossRefPubMedGoogle Scholar
  14. 14.
    Wergedahl H, Liaset B, Gudbrandsen O, et al. Fish protein hydrolysate reduces plasma total cholesterol, increase the proportion of HDL cholesterol and lowers acyl-CoA: Cholesterol acyltransferase activity in liver of Zucher rats. J Nutri, 2004, 134: 1320–1327, 1:CAS:528:DC%2BD2cXkslylu74%3DGoogle Scholar

Copyright information

© Science in China Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  1. 1.College of Life SciencesSouth China Normal University, Guangdong Provincial Key Lab of Biotechnology of Plant DevelopmentGuangzhouChina
  2. 2.Institute of BiophysicsChinese Academy of SciencesBeijingChina

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