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Science in China Series C: Life Sciences

, Volume 51, Issue 11, pp 1020–1024 | Cite as

Purification and characterization of a novel cholesterol-lowering protein from the seeds of Senna obtusifolia

  • ChuHua LiEmail author
  • Mei Li
  • WenRui Chang
  • BaoJiang Guo
Article

Abstract

“Juemingzi”, a source of traditional Chinese herbal medicine, has been demonstrated to play a role in decreasing serum cholesterol concentration. In this study, a novel protein, which has shown an inhibitory effect on cholesterol biosynthesis, was isolated from Senna obtusifolia L. seed by gel filtration and ion exchange chromatography. The novel protein’s molecular mass was 19.7 kD and its pI was 4.80. Both SDS-PAGE and isoelectric-focusing (IEF) revealed a single Coomassie brilliant blue stained band, indicating that the novel protein was a single peptide. The N-terminal amino acid sequence of the protein was IPYISASFPLNIEFLPSE, which had no similarity with any other protein sequences in the NCBI protein database. Circular dichroism (CD) signals indicated that S. obtusifolia seed protein contained 12.5% α-helix, 55.6% β-sheet, and 31.9% random coil

Keywords

Senna obtusifolia L. seed novel protein purification biochemical properties cholesterol biosynthesis 

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Copyright information

© Science in China Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  1. 1.College of Life SciencesSouth China Normal University, Guangdong Provincial Key Lab of Biotechnology of Plant DevelopmentGuangzhouChina
  2. 2.Institute of BiophysicsChinese Academy of SciencesBeijingChina

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