Science in China Series C: Life Sciences

, Volume 51, Issue 7, pp 648–654 | Cite as

KAI1/CD82 suppresses hepatocyte growth factor-induced migration of hepatoma cells via upregulation of Sprouty2

  • ZhenBin Mu
  • Hua Wang
  • Jing Zhang
  • QingFang Li
  • LiSheng Wang
  • XiaoZhong Guo
Article
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Accepted:

Abstract

We conducted a study concerning the suppressive mechanism of KAI1/CD82 on hepatoma cell metastasis. Hepatocyte growth factor (HGF) induces the migration of hepatoma cells through activation of cellular sphingosine kinase 1 (SphK1). Adenovirus-mediated gene transfer of KAI1 (Ad-KAI1) downregulates the SphK1 expression and suppresses the HGF-induced migration of SMMC-7721 human hepatocellcular carcinoma cells. Overexpression of KAI1/CD82 significantly elevates Sprouty2 at the protein level. Ablation of Sprouty2 with RNA interference can block the KAI1/CD82-induced suppression of hepatoma cell migration and downregulation of SphK1 expression. It is demonstrated that KAI1/CD82 suppresses HGF-induced migration of hepatoma cells via upregulation of Sprouty2.

Keywords

KAI1/CD82 Sprouty2 migration hepatocyte growth factor 
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Copyright information

© Science in China Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  • ZhenBin Mu
    • 1
    • 3
  • Hua Wang
    • 2
  • Jing Zhang
    • 2
  • QingFang Li
    • 2
  • LiSheng Wang
    • 2
  • XiaoZhong Guo
    • 3
  1. 1.State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing HospitalFourth Military Medical UniversityXi’anChina
  2. 2.Department of Experimental HematologyBeijing Institute of Radiation MedicineBeijingChina
  3. 3.Department of GastroenterologyShenyang General Hospital of PLAShenyangChina

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