Science in China Series C: Life Sciences

, Volume 51, Issue 1, pp 52–59 | Cite as

Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis

  • Zhang Min 
  • Zhao Cong 
  • Du LianXiang 
  • Lu FuPing 
  • Gao Chen 


The gene coding for a thermophilic neutral protease from Bacillus stearothermophilus was expressed in Bacillus subtilis DB104, under the control of the sacB gene promoter. This was followed by either the native signal peptide sequence of this protease or the signal peptide sequence of the sacB gene. The protease was purified 3.8-fold, with a specific activity of 16530 U mg−1. As analyzed by SDS-PAGE, the molecular mass of the expressed protease was about 35 kDa, and the optimal temperature and pH of the protease were 65°C and 7.5, respectively. Moreover, it still had about 80% activity after 1 h reaction at 65 °C.


Bacillus subtilis thermophilic neutral protease expression purification characterization 


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Copyright information

© Science in China Press 2008

Authors and Affiliations

  • Zhang Min 
    • 1
    • 2
  • Zhao Cong 
    • 2
  • Du LianXiang 
    • 2
  • Lu FuPing 
    • 2
  • Gao Chen 
    • 3
  1. 1.College of EngineeringShenyang Agricultural UniversityShenyangChina
  2. 2.College of BiotechnologyTianjin University of Science and TechnologyTianjinChina
  3. 3.College of Life ScienceNankai UniversityTianjinChina

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