Science in China Series C: Life Sciences

, Volume 50, Issue 2, pp 141–146 | Cite as

Localization of phosphorylated TrkA in carrier vesicles involved in its nuclear translocation in U251 cell line



A number of transmembrane receptors are targeted to the nucleus and convincingly localized therein. However, what remains a conundrum is how these cell-surface receptors end up in the nucleus. In this study, we reported that the transmembrane receptor phosphorylated TrkA was located in a series of carrier vesicles, including ring-like vesicles near the plasma membrane, large core vesicles and small dense core vesicles around the nuclei, as well as in the nucleus in human glioma cell line U251 using immunocytochemistry and immunofluorescence staining. Meanwhile, we also showed that small dense core vesicles budded from large core vesicles, and interacted with the nuclear envelope. Accordingly, our results suggested that such a series of membrane compartments might be involved in the pathway of nuclear translocation of the transmembrane receptor TrkA.


TrkA nuclear translocation vesicular transport large core vesicle small dense core vesicle 


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Copyright information

© Science in China Press 2007

Authors and Affiliations

  • Gong AiHua 
    • 1
  • Zhang ZhiJian 
    • 1
  • Xiao DeSheng 
    • 1
  • Yang Yong 
    • 1
  • Wang YongZhong 
    • 1
  • Chen YongChang 
    • 1
  1. 1.School of MedicineJiangsu UniversityZhenjiangChina

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