Science in China Series C: Life Sciences

, Volume 49, Issue 6, pp 530–542 | Cite as

Isolation and characterization of resistance and defense gene analogs in cotton (Gossypium barbadense L.)

  • Gao Yulong 
  • Guo Wangzhen 
  • Wang Lei 
  • Zhang Tianzhen 
Article
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Abstract

Plant disease resistance gene (R gene) and defense response gene encode some conserved motifs. In the present work, a PCR strategy was used to clone resistance gene analogs (RGAs) and defense gene analogs (DGAs) from Sea-island cotton variety Hai7124 using oligonucleotide primers based on the nucleotide-binding site (NBS) and serine/threonine kinase (STK) in the R-gene and pathogenesis-related proteins of class 2 (PR2) of defense response gene. 79 NBS sequences, 21 STK sequences and 11 DGAs were cloned from disease-resistance cotton. Phylogenic analysis of 79 NBS-RGAs and NBS-RGAs nucleotide sequences of cotton already deposited in GenBank identified one new sub-cluster. The deduced amino acid sequences of NBS-RGAs and STK-RGAs were divided into two distinct groups respectively: Toll/Interleukin-1 receptor (TIR) group and non-TIR group, A group and B group. The expression of RGAs and DGAs having consecutive open reading frame (ORF) was also investigated and it was found that 6 NBS-RGAs and 1 STK-RGA were induced, and 1 DGA was up-regulated by infection of Verticillium dahliae strain VD8. 4 TIR-NBS-RGAs and 4 non-TIR-NBS-RGAs were arbitrarily used as probes for Southern-blotting. There existed 2–10 blotted bands. In addition, since three non-TIR-NBS-RGAs have the same hybridization pattern, we conjecture that these three RGAs form a cluster distribution in the genome.

Keywords

cotton resistance gene analogs (RGAs) defense gene analogs (DGAs) 
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Copyright information

© Science in China Press 2006

Authors and Affiliations

  • Gao Yulong 
    • 1
  • Guo Wangzhen 
    • 1
  • Wang Lei 
    • 1
  • Zhang Tianzhen 
    • 1
  1. 1.National Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina

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