Simultaneous bioimaging of MMP-2 and MMP-7 via Au-Se constructed fluorescence nanoprobe

  • Xiaonan Gao
  • Wenfei Guo
  • Lulu Jiang
  • Bo Hu
  • Xiaojun Liu
  • Kehua XuEmail author
  • Bo TangEmail author


Au-Se bond strategy has been applied to construct fluorescence nanoprobe that is being used to simultaneously dual-image the tumor markers matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-7 (MMP-7) in vitro. The two Se-terminating ligand peptide chains respectively labeled with fluorescein isothiocyanate (FITC) and 5-Carboxytetramethylrhodamine (5-TAMRA) dyes are attached to the surface of Au nanoparticles (NPs). The nanoprobe can be specifically cut off by MMP-2 and MMP-7 respectively to carry out significant enhanced fluorescence recovery for simultaneously imaging both markers. The nanoprobe not only displays high selectivity and sensitivity towards MMP-2 and MMP-7, but also has strong anti-interference stability against biothiols and enhanced fidelity for avoiding ‘false positive’ results. This approach offers new perspectives in designing tumor-related early diagnosis approaches and also provides strategies for clinical applications.


fluorescence nanoprobe dual detection tumor markers fluorescence imaging Au nanoparticles 


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This work was supported by the National Natural Science Foundation of China (21535004, 91753111, 21575081, 21775091, 21705098), the Key Research and Development Program of Shandong Province (2018YFJH0502), the Natural Science Foundation of Shandong Province (ZR201702200428) and the Project of Shandong Province Higher Educational Science and Technology Program (J17KA116).

Supplementary material

11426_2019_9646_MOESM1_ESM.docx (4.6 mb)
Simultaneous Bioimaging of MMP-2 and MMP-7 via Au-Se Constructed Fluorescence Nanoprobe


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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of ShandongShandong Normal UniversityJinanChina

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