Single-molecule analysis in an electrochemical confined space
- 169 Downloads
Electrochemical analysis of single molecules is a method with the strong ability of the enhanced efficiency and ultra-sensitivity. Here, we demonstrate that the electrochemical confined space could efficiently convert single molecule characteristics into measurable electrochemical signatures with high temporal resolution. The human telomere repeat sequence T8 was used as a probe to determine the electrochemical confined effect in a nanopore. Our results show that the nanopore with comparable confined space of the telomere repeat sequence exhibits the most distinguishable single-molecule signals which suggest the folded conformation of T8. This method will greatly extend the lifetime of a metastable conformation for a single biomolecule by strong analyte-nanopore interactions, which brings the new insight into the understanding of the biomolecule’s function at single-molecule level.
Keywordselectrochemical confined space nanopores single-molecule analysis DNAs
Unable to display preview. Download preview PDF.
This work was supported by the National Natural Science Foundation of China (21421004, 21505043, 21327807), and the Fundamental Research Funds for the Central Universities (222201718001, 222201717003, 222201714012).