Science China Chemistry

, Volume 60, Issue 9, pp 1187–1190 | Cite as

Single-molecule analysis in an electrochemical confined space



Electrochemical analysis of single molecules is a method with the strong ability of the enhanced efficiency and ultra-sensitivity. Here, we demonstrate that the electrochemical confined space could efficiently convert single molecule characteristics into measurable electrochemical signatures with high temporal resolution. The human telomere repeat sequence T8 was used as a probe to determine the electrochemical confined effect in a nanopore. Our results show that the nanopore with comparable confined space of the telomere repeat sequence exhibits the most distinguishable single-molecule signals which suggest the folded conformation of T8. This method will greatly extend the lifetime of a metastable conformation for a single biomolecule by strong analyte-nanopore interactions, which brings the new insight into the understanding of the biomolecule’s function at single-molecule level.


electrochemical confined space nanopores single-molecule analysis DNAs 


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This work was supported by the National Natural Science Foundation of China (21421004, 21505043, 21327807), and the Fundamental Research Funds for the Central Universities (222201718001, 222201717003, 222201714012).

Supplementary material

11426_2017_9082_MOESM1_ESM.pdf (124 kb)
Single-molecule analysis in an electrochemical confined space


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Copyright information

© Science China Press and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Key Laboratory for Advanced Materials & School of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghaiChina

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