Science China Chemistry

, Volume 59, Issue 4, pp 493–496 | Cite as

Colorimetric detection and efficient monitoring of a potential biomarker of lumbar disc herniation using carbon nanotube-based probe

  • Yufeng Huang
  • Ju Liu
  • Min Zou
  • Qingzhi Zhang
  • Jie Chao
  • Weidong Zhao
  • Desheng Wu
  • Shao Su
  • Lianhui Wang
Articles
  • 41 Downloads

Abstract

Herein, a multiwalled carbon nanotubes (MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1 (ApoL1) in lumbar disc herniation (LDH) patients. ApoL1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group (Ctrl), spine spondylolisthesis (SSP) group, spinal fracture (SFR) group, and spine scoliosis (SSC) group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was <0.001 and >0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay (ELISA), respectively, suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that ApoL1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a potential application in discovering new biomarkers of diseases.

Keywords

colorimetric probe apolipoprotein-L1 early diagnosis recovery monitoring 

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Yufeng Huang
    • 1
  • Ju Liu
    • 2
  • Min Zou
    • 3
  • Qingzhi Zhang
    • 1
  • Jie Chao
    • 3
  • Weidong Zhao
    • 1
  • Desheng Wu
    • 1
  • Shao Su
    • 3
  • Lianhui Wang
    • 3
  1. 1.Department of Spine Surgery, Shanghai East HospitalTongji UniversityShanghaiChina
  2. 2.Department of Orthopedic SurgeryShanghai Pudong New District Zhoupu HospitalShanghaiChina
  3. 3.Key Laboratory for Organic Electronics & Information Displays, Institute of Advanced Materials; National Syngerstic Innovation Center for Advanced MaterialsNanjing University of Posts & TelecommunicationsNanjingChina

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