Science China Chemistry

, Volume 59, Issue 4, pp 487–492 | Cite as

Fluorescent carbon dots derived from lactose for assaying folic acid

  • Zhangbao Chen
  • Jing Wang
  • Hong Miao
  • Lan Wang
  • Shuang Wu
  • Xiaoming Yang
Articles

Abstract

The fluorescent carbon dots were successfully synthesized by simply heating the mixture of lactose and NaOH solution. The as-synthesized carbon dots had been systematically characterized by fluorescence, Fourier transform infrared (FTIR), high resolution transmission electron microscopy (HR-TEM) and 13C NMR. Since the fluorescence of the carbon dots was efficiently quenched by folic acid, the carbon dots were employed as selective fluorescence probes for detecting folic acid, depending on the formation of hydrogen bond among the functional group of folic acid (–OH,–COOH and–NH2) and–OH and–COOH of the carbon dots. Moreover, the decrease of fluorescence intensity was capable of detecting folic acid in a linear range of 6×10-5–8×10-8 mol/L with a detection limit of 1.2×10-9 mol/L at a signal-to-noise ratio of 3, suggesting a promising assay for folic acid. Significantly, the practicability of this fluorescence probe to assay folic acid in human urine samples was further evaluated.

Keywords

carbon quantum dots fluorescence quenching 

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Zhangbao Chen
    • 1
  • Jing Wang
    • 1
  • Hong Miao
    • 1
  • Lan Wang
    • 1
  • Shuang Wu
    • 1
  • Xiaoming Yang
    • 1
  1. 1.College of Pharmaceutical SciencesSouthwest UniversityChongqingChina

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