Science China Chemistry

, Volume 59, Issue 4, pp 487–492 | Cite as

Fluorescent carbon dots derived from lactose for assaying folic acid

  • Zhangbao Chen
  • Jing Wang
  • Hong Miao
  • Lan Wang
  • Shuang Wu
  • Xiaoming Yang


The fluorescent carbon dots were successfully synthesized by simply heating the mixture of lactose and NaOH solution. The as-synthesized carbon dots had been systematically characterized by fluorescence, Fourier transform infrared (FTIR), high resolution transmission electron microscopy (HR-TEM) and 13C NMR. Since the fluorescence of the carbon dots was efficiently quenched by folic acid, the carbon dots were employed as selective fluorescence probes for detecting folic acid, depending on the formation of hydrogen bond among the functional group of folic acid (–OH,–COOH and–NH2) and–OH and–COOH of the carbon dots. Moreover, the decrease of fluorescence intensity was capable of detecting folic acid in a linear range of 6×10-5–8×10-8 mol/L with a detection limit of 1.2×10-9 mol/L at a signal-to-noise ratio of 3, suggesting a promising assay for folic acid. Significantly, the practicability of this fluorescence probe to assay folic acid in human urine samples was further evaluated.


carbon quantum dots fluorescence quenching 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Prato M. J Mater Chem, 1997, 7: 1097–1109CrossRefGoogle Scholar
  2. 2.
    Yang ST, Cao L, Luo PG, Lu F, Wang X, Wang H, Meziani MJ, Liu Y, Qi G, Sun YP. J Am Chem Soc, 2009, 131: 11308CrossRefGoogle Scholar
  3. 3.
    He DH, Zheng CB, Wang Q, He CL, Lee YI, Wu L, Hou XD. Talanta, 2015, 142: 51–56CrossRefGoogle Scholar
  4. 4.
    Bourlinos AB, Stassinopoulos A, Anglos D, Zboril R, Karakassides M, Giannelis EP. Small, 2008, 4: 455–458CrossRefGoogle Scholar
  5. 5.
    Ploehn HJ, Gu YL, Xu XY, Raker K, Gearheart L, Ray R, Scrivens WA. J Am Chem Soc, 2004, 126: 12736–12737CrossRefGoogle Scholar
  6. 6.
    Cao L, Sahu S, Anilkumar P, Bunker CE, Xu J, Shiral Fernando KA, Wang P, Guliants EA, Tackett II KN, Sun YP. J Am Chem Soc, 2011, 133: 4754–4757CrossRefGoogle Scholar
  7. 7.
    Li HT, He XD, Kang ZH, Huang H, Liu Y, Liu JI, Lian SY, Tsang CHA, Yang XB, Lee ST. Angew Chem Int Ed, 2010, 49: 4430–4434CrossRefGoogle Scholar
  8. 8.
    Cao L, Wang X, Meziani MJ, Lu F, Wang H, Luo PJG, Lin Y, Harruff BA, Veca LM, Murray D, Xie SY, Sun YP. J Am Chem Soc, 2007, 129: 11318–11319CrossRefGoogle Scholar
  9. 9.
    Guo YM, Wang Z, Shao HW, Jiang XY. Carbon, 2013, 52: 583–589CrossRefGoogle Scholar
  10. 10.
    Pan D, Zhang J, Li Z, Wu C, Yan X, Wu M. Chem Commun, 2010, 46: 3681–3683CrossRefGoogle Scholar
  11. 11.
    Zhu H, Wang X, Li Y, Wang Z, Yang F, Yang X. Chem Commun, 2009, 34: 5118–5120CrossRefGoogle Scholar
  12. 12.
    Zheng L, Chi Y, Dong Y, Lin J, Wang B. J Am Chem Soc, 2009, 131: 4564–4565CrossRefGoogle Scholar
  13. 13.
    Sun YP, Zhou B, Lin Y, Wang W, Fernando KA, Pathak P, Meziani MJ, Harruff BA, Wang X, Wang H, Luo PG, Yang H, Kose ME, Chen B, Veca LM, Xie SY. J Am Chem Soc, 2006, 128: 7756–7757CrossRefGoogle Scholar
  14. 14.
    Liu H, Ye T, Mao CD. Angew Chem Int Ed, 2007, 46: 6473–6475CrossRefGoogle Scholar
  15. 15.
    Sahu S, Behera B, Maiti TK, Mohapatra S. Chem Commun, 2012, 48: 8835–8837CrossRefGoogle Scholar
  16. 16.
    Wei SH, Zhao FQ, Xu ZY, Zeng BZ. Microchim Acta, 2006, 152: 285–290CrossRefGoogle Scholar
  17. 17.
    Hoegger D, Morier P, Vollet C, Heini D, Reymond F, Rossier JS. Anal Bioanal Chem, 2007, 387: 267–275CrossRefGoogle Scholar
  18. 18.
    Nelson BC, Sharpless KE, Sander LC. J Chromatogr A, 2006, 1135: 203–211CrossRefGoogle Scholar
  19. 19.
    Zhao S, Yuan H, Xie C, Xiao D. J Chromatogr A, 2006, 1107: 290–293CrossRefGoogle Scholar
  20. 20.
    Aurora-Pradoa MS, Silvaa CA, Tavaresa MFM, Altriab KD. J Chromatogr A, 2004, 1051: 291–296CrossRefGoogle Scholar
  21. 21.
    Rodriguez-Bernaldo de Quiros A, Castro de Ron C, Lopez-Hernandez J, Lage-Yusty MA. J Chromatogr A, 2004, 1032: 135–139CrossRefGoogle Scholar
  22. 22.
    Li, XH, Zhao ZW. Rsc Adv, 2014, 4: 57615–57619CrossRefGoogle Scholar
  23. 23.
    Cao HJ, Liu SS, Tu WW, Bao JC, Dai ZH. Chem Commun, 2014, 50: 13315–13318CrossRefGoogle Scholar
  24. 24.
    Yang MM, Li H, Liu J, Kong WQ, Zhao SY, Li CX, Huang H, Li Y, Kang ZH. J Mater Chem B, 2014, 2: 7964–7970CrossRefGoogle Scholar

Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Zhangbao Chen
    • 1
  • Jing Wang
    • 1
  • Hong Miao
    • 1
  • Lan Wang
    • 1
  • Shuang Wu
    • 1
  • Xiaoming Yang
    • 1
  1. 1.College of Pharmaceutical SciencesSouthwest UniversityChongqingChina

Personalised recommendations