Science China Chemistry

, Volume 57, Issue 12, pp 1696–1702 | Cite as

Logic-signal output of fluorescent proteins for screening antibiotic combinations

Articles Special Issue Biophysical Chemistry
First Online:
Received:
Accepted:

Abstract

A new method to screen antibiotic combinations is demonstrated, which takes advantage of the logic-signal output of genetically engineered drug-resistant E. coli strains expressing different fluorescent proteins. Thirty-six antibiotic combinations for nine antibiotics were investigated. The operation of different logic gates can reveal the susceptibility, resistance, or synergistic effect of the antibiotic combinations in a rapid (7–8 h versus 24–28 h for typical growth-based assays), simple, quantitative and high-throughput manner. This logic-signal-based output patterns provide the basis for novel and reliable screening of antibiotic combinations and help us to both gain insight into the mechanisms of multi-drug action.

Keywords

fluorescent proteins drug-resistance bacteria antibiotic screening logic signal 
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Supplementary material

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of ChemistryChinese Academy of SciencesBeijingChina

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