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Science China Chemistry

, Volume 56, Issue 12, pp 1701–1709 | Cite as

Preparation of enzymatically cross-linked sulfated chitosan hydrogel and its potential application in thick tissue engineering

  • ZhiPing Chen
  • Wei WangEmail author
  • Lei Guo
  • YanYan Yu
  • Zhi YuanEmail author
Articles

Abstract

For the requirement of preliminary vascularization, the scaffolds for thick tissue engineering should possess not only good cell affinity, but also anticoagulant ability. In this paper, an enzymatically crosslinked hydrogel scaffold based on sulfated chitosan (SCTS) was prepared. Firstly, sulfated chitosan-hydroxyphenylpionic acid (SCTS-HPA) conjugate was synthesized, and its structure was identified by FITR and 1H NMR. And then an enzymatically crosslinked hydrogel was prepared in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The gelation time, mechanical property, morphology and cytotoxicity to human umbilical vein endothelial cells (HUVECs) of the hydrogel was evaluated in vitro, the tissue compatibility of SCTS scaffold was studied in vivo. The results showed that the gelation time, mechanical property, morphology of the dehydrated hydrogel could be controlled by the HRP and H2O2 concentration. The cytotoxicity test showed that the hydrogel extracts had no cytotoxicity to HUVECs. The in vivo assay indicated that SCTS-HPA scaffold showed good tissue compatibility, and no thrombus formation. All these results indicated that the SCTS-HPA scaffold could be used as thick tissue engineering scaffold.

Keywords

enzymatically crosslinked thick tissue engineering scaffold thrombus formation tissue compatibility 

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Key Laboratory of Functional Polymer Materials, Ministry of Education; Institute of Polymer ChemistryNankai UniversityTianjinChina

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