Science China Chemistry

, 54:1298 | Cite as

Immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence for the detection of abscisic acid

  • GuoHua Zhou
  • Ping Wang
  • Ju Yuan
  • Ting Qiu
  • ZhiKe He
Articles
First Online:
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Accepted:

Abstract

Abscisic acid (ABA) is an important plant hormone. It plays a key role in regulating plant responses to abiotic stress and in controlling seed germination, growth, and stomatal aperture. A rapid, sensitive analytical method for the ABA detection is urgently required for further investigation of ABA signaling. In this work, an immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence has been developed for the detection of ABA. The result could be read out in 30 min at least, with the simplified procedure of immunomagnetic assay. Under the optimized condition, a linear range from 1 pM to 10 nM was obtained. An unexpected result induced by the dose hook effect was discussed. This method provided the high selectivity for ABA over other components that might be contained in real samples.

Keywords

abscisic acid immunomagnetic assay chemiluminescence CdSe/ZnS 
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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • GuoHua Zhou
    • 1
  • Ping Wang
    • 1
  • Ju Yuan
    • 1
  • Ting Qiu
    • 1
  • ZhiKe He
    • 1
  1. 1.Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education; College of Chemistry and Molecular SciencesWuhan UniversityWuhanChina

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