Science China Chemistry

, Volume 53, Issue 4, pp 820–825

Immobilization of acetylcholinesterase on one-dimensional gold nanoparticles for detection of organophosphorous insecticides

Articles

Abstract

This paper reports a simple method for immobilization of acetylcholinesterase (AChE) on one-dimensional (1D) gold (Au) nanoparticles for detection of organophosphorous (OP) insecticides. 1D Au nanoparticles were prepared by electrodeposition in the pores of an alumina template which was subsequently removed by 2.0 M NaOH solution. They were characterized by XRD and FESEM. The immobilized AChE retained its biological activity and catalyzed the hydrolysis of acetylthiocholine to form thiocholine, which was subsequently oxidized to produce detectable signals. Based on the inhibition toward the enzymatic activity of AChE by OP insecticides, sensitive detection of methamidophos (an OP insecticide) was performed. Under optimal conditions, the sensors could be used for the determination of methamidophos ranging from 0.004 to 24 μg/mL with the detection limit of 0.001 μg/mL. The developed OP insecticide biosensors exhibited satisfactory stability and reproducibility. This work demonstrated that 1D Au nanoparticles could serve as an ideal carrier for immobilization of AChE to fabricate the corresponding biosensor.

Keywords

acetylcholinesterase organophosphorous insecticides immobilization gold 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kumar J, Jha SK, D’souza SF. Optical microbial biosensor for detection of methyl parathion pesticide using flavobacterium sp. whole cells adsorbed on glass fiber filters as disposable biocomponent. Biosens Bioelectron, 2006, 21: 2100–2105Google Scholar
  2. 2.
    Rotiroti L, Stefano LD, Rendina I, Moretti L. Optical microsensors for pesticides identification based on porous silicon technology. Biosens Bioelectron, 2005, 20: 2136–2139CrossRefGoogle Scholar
  3. 3.
    Leandro CC, Hancock P, Fussell RJ, Keely BJ. Comparison of ultra-performance liquid chromatography and high-performance liquid chromatography for the determination of priority pesticides in baby foods by tandem quadrupole mass spectrometry. J Chromatogr A, 2006, 1103: 94–101CrossRefGoogle Scholar
  4. 4.
    Amine A, Mohammadi H, Bourais I, Palleschi G. Enzyme inhibition-based biosensors for food safety and environmental monitoring. Biosens Bioelectron, 2006, 21: 1405–1423CrossRefGoogle Scholar
  5. 5.
    Du D, Huang X, Cai J, Zhang AD. Amperometric detection of triazophos pesticide using acetylcholinesterase biosensor based on multiwall carbon nanotube-chitosan matrix. Sens Actuators B, 2007, 127: 531–535CrossRefGoogle Scholar
  6. 6.
    Liu GD, Riechers SL, Mellen MC, Lin YH. Sensitive electrochemical detection of enzymatically generated thiocholine at carbon nanotube modified glassy carbon electrode. Electrochem Commun, 2005, 7: 1163–1169CrossRefGoogle Scholar
  7. 7.
    Xiao Y, Patolsky F, Katz E, Hainfeld JF, Willner I. ’Plugging into enzymes: nanowiring of redox enzymes by a gold nanoparticle. Science, 2003, 299: 1877–1881CrossRefGoogle Scholar
  8. 8.
    Luo XL, Xu JJ, Du Y, Chen HY. A glucose biosensor based on chitosan-glucose oxidase-gold nanoparticles biocomposite formed by one-step electrodeposition. Anal Biochem, 2004, 334: 284–289CrossRefGoogle Scholar
  9. 9.
    Dagan-Moscovich H, Cohen-Hadar N, Porat C, Rishpon J, Shacham-Diamand Y, Freeman A. Nanowiring of the catalytic site of novel molecular enzyme-metal hybrids to electrodes. J Phys Chem C, 2007, 111: 5766–5769CrossRefGoogle Scholar
  10. 10.
    Zhou H, Chen H, Luo S, Chen J, Wei W, Kuang Y. Glucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline. Biosens Bioelectron, 2005, 20: 1305–1311CrossRefGoogle Scholar
  11. 11.
    Pan D, Chen J, Yao S, Nie L, Xia J, Tao W. Amperometric glucose biosensor based on immobilization of glucose oxidase in electropolymerized o-aminophenol film at copper-modified gold electrode. Sens Actuators B, 2005, 104: 68–74CrossRefGoogle Scholar
  12. 12.
    Liu S, Leech D, Ju H. Application of colloidal gold in protein immobilization, electron transfer, and biosensing. Anal Lett, 2003, 36: 1–19CrossRefGoogle Scholar
  13. 13.
    Du D, Chen SZ, Cai J, Zhang AD. Electrochemical pesticide sensitivity test using acetylcholinesterase biosensor based on colloidal gold nanoparticle modified sol-gel interface. Talanta, 2008, 74: 766–772CrossRefGoogle Scholar
  14. 14.
    Du D, Ding JW, Cai J, Zhang AD. Electrochemical thiocholine inhibition sensor based on biocatalytic growth of Au nanoparticles using chitosan as template. Sens and Actuators B, 2007, 127: 317–322CrossRefGoogle Scholar
  15. 15.
    Du D, Chen SZ, Cai J, Zhang AD. Electrochemical pesticide sensitivity test using acetylcholinesterase biosensor based on colloidal gold nanoparticle. Talanta, 2008, 74: 766–772CrossRefGoogle Scholar
  16. 16.
    Du D, Ding JW, Cai J, Zhang AD. One-step electrochemically deposited interface of chitosan-gold nanoparticles for acetylcholinesterase biosensor design. J Electroanal Chem, 2007, 605: 53–60CrossRefGoogle Scholar
  17. 17.
    Kolmakov A, Zhang YX, Cheng GS, Moskovits M. Detection of CO and O2 using tin oxide nanowire sensors. Adv Mater, 2003, 15: 997–1000CrossRefGoogle Scholar
  18. 18.
    Heins EA, Siwy ZS, Baker LA, Martin CR. Detecting single porphyrin molecules in a conically shaped synthetic nanopore. Nano Lett, 2005, 5: 1824–1829CrossRefGoogle Scholar
  19. 19.
    Liu LL, Bao JC, Fang M, Li LF, Dai ZH. Electrogenerated chemiluminescence for the sensitive detection of leucine using Ru(bpy)3 2+ immobilized on dendritic Pd nanoparticle. Sens Actuators B, 2009, 139: 527–531CrossRefGoogle Scholar
  20. 20.
    Bao JC, Xu DP, Zhou QF, Xu Z, Feng YY, Zhou YM. An array of concentric composite nanostructure of metal nanowires encapsulated in zirconia nanotubes: preparation, characterization, and magnetic properties. Chem Mater, 2002, 14: 4709–4713CrossRefGoogle Scholar
  21. 21.
    Kauppinen JK, Moffatt DJ, Mantsch HH, Cameron DG. Resolution of complex band contours by means of Fourier self-deconvolution. Appl Spectrosc, 1981, 35: 271–276CrossRefGoogle Scholar
  22. 22.
    Dong S, Luo GA, Feng J, Li QW, Gao H. Immunoassay of staphylococcal enterotoxin C1 by FTIR spectroscopy and electrochemical gold electrode. Electroanalysis, 2001, 13: 30–33CrossRefGoogle Scholar
  23. 23.
    Kandimalla VB, Ju HX. Binding of acetylcholinesterase to multiwall carbon nanotube-cross-linked chitosan composite for flow-injection amperometric detection of an organophosphorous insecticide. Chem Eur J, 2006, 12: 1074–1080CrossRefGoogle Scholar
  24. 24.
    Du D, Ding JW, Cai J, Zhang AD. Determination of carbaryl pesticide using amperometric acetylcholinesterase sensor formed by electrochemically deposited chitosan. Colloid Surf B, 2007, 58: 145–150CrossRefGoogle Scholar
  25. 25.
    Delfino RT, Figueroa-Villar JD. Nucleophilic reactivation of sarin-inhibited a cetylcholinesterase: a molecular modeling study. J Phys Chem B, 2009, 113: 8402–8411CrossRefGoogle Scholar

Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Jiangsu Key Laboratory of Biofunctional MaterialsNanjing Normal UniversityNanjingChina
  2. 2.College of Chemistry and Environmental ScienceNanjing Normal UniversityNanjingChina

Personalised recommendations