Analysis of human urine metabolites using SPE and NMR spectroscopy

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Nuclear magnetic resonance (NMR) spectroscopic analysis of metabonome/metabolome has wide-spread applications in biomedical science researches. However, most of NMR resonances for urinary metabolites remain to be fully assigned. In the present study, human urine samples from two healthy volunteers were pre-treated with C18 solid-phase extraction and the resultant 5 sub-fractions were subjected to one-and two-dimensional NMR studies, including 1H J-Resolved, 1H-1H COSY, 1H-1H TOCSY, 1H-13C HSQC, and HMBC 2D NMR. More than 70 low molecular weight metabolites were identified, and complete assignments of 1H and 13C resonances including many complex coupled spin systems were obtained.

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Author information

Correspondence to WeiJin Yang or HuiRu Tang.

Additional information

Supported by the Chinese Academy of Sciences (Grant No: KZCX2-YW-420-21), the National High Technology Research and Development Program of China (Grant No: 2006AA06Z424), and the National Natural Science Foundation of China (Grant Nos: 40590392 and 20575074)

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Yang, W., Wang, Y., Zhou, Q. et al. Analysis of human urine metabolites using SPE and NMR spectroscopy. Sci. China Ser. B-Chem. 51, 218–225 (2008).

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  • urine metabolites
  • NMR spectroscopy
  • solid phase extraction
  • metabonomics/metabolomics