Science in China Series B: Chemistry

, Volume 51, Issue 3, pp 218–225 | Cite as

Analysis of human urine metabolites using SPE and NMR spectroscopy

Article

Abstract

Nuclear magnetic resonance (NMR) spectroscopic analysis of metabonome/metabolome has wide-spread applications in biomedical science researches. However, most of NMR resonances for urinary metabolites remain to be fully assigned. In the present study, human urine samples from two healthy volunteers were pre-treated with C18 solid-phase extraction and the resultant 5 sub-fractions were subjected to one-and two-dimensional NMR studies, including 1H J-Resolved, 1H-1H COSY, 1H-1H TOCSY, 1H-13C HSQC, and HMBC 2D NMR. More than 70 low molecular weight metabolites were identified, and complete assignments of 1H and 13C resonances including many complex coupled spin systems were obtained.

Keywords

urine metabolites NMR spectroscopy solid phase extraction metabonomics/metabolomics 

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Copyright information

© Science in China Press 2008

Authors and Affiliations

  1. 1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina
  2. 2.State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhanChina

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