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Assessment of the role played by n-propanol in distinction of ethanol source in postmortem blood with the assistance of ethyl glucuronide and ethyl sulfate

  • Hao Wang
  • Jiaolun Li
  • Zhibin Huang
  • Fanglin Wang
  • Yunfeng Zhang
  • Jing Chang
  • Yulan RaoEmail author
Original Article

Abstract

Purpose

Concentration ratio of ethanol/n-propanol has been employed to distinguish the source of ethanol in postmortem blood, though its reliability remains controversial.

Methods

Forty-two postmortem human blood samples with ethanol levels in the range of 0.07–4.64 mg/mL were investigated. Ethanol and n-propanol were determined by head-space gas chromatography coupled with flame ionization detection, while ethyl glucuronide (EtG) and ethyl sulfate (EtS) were determined by liquid chromatography–tandem mass spectrometry.

Results

EtG and EtS were both negative in 26% of the investigated postmortem blood samples and 11% of n-propanol-negative postmortem samples, indicating that n-propanol was not a reliable marker of putrefaction. It was also found that the ratio of ethanol/n-propanol (supposed to be < 20 without antemortem ethanol consumption) was unreliable by showing great individual differences and was opposite with the result of EtG and EtS in at least 17% of n-propanol-positive postmortem blood samples. Meanwhile, 140 antemortem blood samples were investigated, as an aid to estimate the blood alcohol concentration (BAC) at the time of death for postmortem samples with ethanol both from postmortem formation and antemortem consumption. By comparing with the maximum or minimum value of EtG and EtS concentration under certain BAC in antemortem samples, the BAC range at the moment of death could be estimated in 93% of postmortem samples.

Conclusions

The present study proved that n-propanol was not a reliable marker for either putrefaction or ethanol source distinction by showing considerable false rate.

Keywords

Ethanol Postmortem n-Propanol Ethyl glucuronide Ethyl sulfate 

Notes

Acknowledgements

This research was supported by Science and Technology Commission of Shanghai Municipality Technical Standards Special Projects (no. 17DZ22204100) and National Key R&D Program of China (no. 2017YFC0803504-2).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Use of blood samples in this study was approved by Ethics Committee of School of Basic Medical Sciences, Fudan University.

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Copyright information

© Japanese Association of Forensic Toxicology 2019

Authors and Affiliations

  1. 1.Department of Forensic Medicine, School of Basic Medical SciencesFudan UniversityShanghaiPeople’s Republic of China
  2. 2.Institute of Forensic ScienceMinistry of Public SecurityBeijingPeople’s Republic of China

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