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Forensic Toxicology

, Volume 37, Issue 1, pp 90–103 | Cite as

Detection of in utero ethanol exposure via ethyl glucuronide and ethyl sulfate analysis in umbilical cord and placenta

  • Jennifer Hanna
  • Elena Lendoiro
  • Ana de Castro
  • Eva Gónzalez-Colmenero
  • Ana Concheiro-Guisan
  • Patricia Peñas-Silva
  • Manuel Macias-Cortiña
  • Angelines Cruz-Landeira
  • Manuel López-Rivadulla
  • Marta Concheiro-GuisanEmail author
Original Article

Abstract

Purpose

Alcohol exposure during pregnancy constitutes one of the leading preventable causes of birth defects, mental retardation and neurodevelopmental disorders in the exposed children. The ethanol marker ethyl glucuronide (EtG) is a specific long-term marker of ethanol in utero exposure in meconium; however, currently, there are scarce or no data about EtG and ethyl sulfate (EtS) in umbilical cord and placenta. These tissues are alternative matrices to meconium that offer critical advantages, such as always being available at birth with noninvasive and easy collection.

Methods

We developed and validated a method for the determination of EtG and EtS in umbilical cord and placenta. Tissues were homogenized in methanol, extracted using weak anion-exchange solid-phase extraction (SPE) and analyzed by liquid chromatography–tandem mass spectrometry. The umbilical cord and placenta method was applied to 59 authentic samples from newborns whose meconium samples were positive for EtG (EtG > 5 ng/g).

Results

The method in umbilical cord and placenta was fully validated, with a limit of quantification at 5 ng/g in umbilical cord and 10 ng/g in placenta for both compounds. EtG and/or EtS were detected in 25 umbilical cord samples (4.4–529 and 4.3–39 ng/g, respectively) and in 8 placenta samples (26.5–267 and 11–24.3 ng/g, respectively). EtG and EtS showed a homogenous distribution throughout umbilical cord tissue (n = 5).

Conclusions

We developed and validated a sensitive and specific method for the determination of EtG and EtS in umbilical cord and placenta. To date, this is the first method to investigate both direct metabolites of ethanol in umbilical cord and placenta samples for prenatal ethanol exposure.

Keywords

Ethyl glucuronide Ethyl sulfate Umbilical cord Placenta Meconium LC–MS/MS 

Notes

Acknowledgements

This project was funded by the Plan Nacional Sobre Drogas, Ministerio de Sanidad, Gobierno de España, grant 2016I005. E. Lendoiro would like to thank the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia, for her postdoctoral contract (ED481B2016/103-0).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by the Ethics Committee of the University of Santiago de Compostela (Spain). All procedures performed in this study were in accordance with the ethical standards of the Ethics Committee of the University of Santiago de Compostela (Spain) and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Copyright information

© Japanese Association of Forensic Toxicology and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Jennifer Hanna
    • 1
  • Elena Lendoiro
    • 2
  • Ana de Castro
    • 2
  • Eva Gónzalez-Colmenero
    • 3
  • Ana Concheiro-Guisan
    • 3
  • Patricia Peñas-Silva
    • 4
  • Manuel Macias-Cortiña
    • 4
  • Angelines Cruz-Landeira
    • 2
  • Manuel López-Rivadulla
    • 2
  • Marta Concheiro-Guisan
    • 1
    Email author return OK on get
  1. 1.John Jay College of Criminal JusticeCity University of New YorkNew YorkUSA
  2. 2.Sección de Toxicología, Instituto de Ciencias ForensesUniversidad de Santiago de CompostelaSantiago de CompostelaSpain
  3. 3.Sección de Neonatología, Complejo HospitalarioUniversitario de VigoVigoSpain
  4. 4.Sección de Ginecología y Obstetricia, Hospital ClínicoUniversitario de Santiago de CompostelaSantiago de CompostelaSpain

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