Abstract
Purpose
The stability of NBOMes, potent new psychoactive substances, in whole blood was studied over 180 days.
Method
We present a fully validated liquid chromatography–tandem mass spectrometry method to quantify seven NBOMes (25B-, 25C-, 25D-, 25E-, 25G-, 25H- and 25I-NBOMe) in whole blood, and a 180 day stability at two analyte concentrations and three storage temperatures [room temperature (RT), 4 °C and −20 °C].
Results
Calibration curves were linear over 0.1–10 ng/mL, with intra- and interday imprecision (% relative standard deviation) and bias (%) not greater than 8.1 %. For low concentration (0.3 ng/mL) samples at RT, 25B-, 25C-, 25I- and 25E-NBOMe decreased by more than 20% after 15 days and were undetectable after 30 days. All NBOMes were below the limit of quantification (LOQ; 0.1 ng/mL) when stored at RT for 60 days. At 4 °C, 25B-, 25C-, 25I-, 25G-, 25D- and 25E-NBOMe (0.3 ng/mL) decreased by more than 20% (up to 54%) after 180 days. Except for 25H-NBOMe, all high concentration (8 ng/mL) NBOMes decreased by more than 20% after 15 days at RT, and high concentrations of 25B-, 25C-, and 25I-NBOMe were unstable at 4 °C after 180 days. All analytes were stable for 180 days at −20 °C. Extracts of low, medium and high quality control samples were stable when reinjected after 32 h storage on the autosampler at 15 °C. All analytes were stable after three freeze-thaw cycles at −20 °C.
Conclusions
Based on the potential for NBOMe instability prior to testing, it is recommended to refrigerate whole blood for NBOMe analysis up to 90 days, and to freeze samples for longer term storage.
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Acknowledgements
The authors thank Fundação de Amparo à Pesquisa do Estado de São Paulo–FAPESP (Process Number 2015/10650-8 and 2018/00432-1), Conselho Nacional de Desenvolvimento Científico e Tecnológico–CNPq (Process Number 131780/2017-4), and Superintendência da Polícia Técnico-Científica for kind donation of authentic samples.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the University of Campinas committee (Comitê de Ética em Pesquisa da UNICAMP—CEP, CAAE 8452716.2.0000.5404 and Superintendência da Polícia Técnico-Científca, ofício No. 766/2015/ATS/SPTC-SSP).
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da Cunha, K.F., Eberlin, M.N., Huestis, M.A. et al. NBOMe instability in whole blood. Forensic Toxicol 37, 82–89 (2019). https://doi.org/10.1007/s11419-018-0438-5
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DOI: https://doi.org/10.1007/s11419-018-0438-5