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Forensic Toxicology

, Volume 37, Issue 1, pp 245–249 | Cite as

Fatal intoxication with 1,1-difluoroethane (DFE) due to inhalation of a spray cleaner: analysis by GC–MS

  • Suguru TorimitsuEmail author
  • Yusuke Fujii
  • Kanju Saka
  • Hiroko Abe
  • Yohsuke Makino
  • Fumiko Chiba
  • Hirotaro Iwase
Case Report

Abstract

Purpose

Animal studies suggested that halogenated hydrocarbons such as 1,1-difluoroethane (DFE) sensitized myocardial tissues to catecholamines and might cause fatal arrhythmia. In this paper, we report a case of a fatality that was associated with DFE abuse, and quantified DFE concentrations in postmortem specimens using gas chromatography–mass spectrometry (GC–MS).

Methods

Femoral vein blood, cardiac blood, and urine samples were taken from the autopsy for toxicological analysis. We have established a detailed procedure for quantification of DFE in human blood and urine by GC–MS and have presented its validation data.

Results

The concentrations of DFE in this case were 481, 591 and 201 µg/mL in femoral vein blood, cardiac blood and urine samples, respectively, which were much higher than those in previous cases measured by gas chromatography–flame ionization detection. Thus, in the absence of other remarkable autopsy findings, the cause of death was determined to be DFE intoxication.

Conclusions

To the best of our knowledge, this is the first case report of quantification of DFE in human blood and urine specimens by GC–MS.

Keywords

1,1-Difluoroethane (DFE) Fatal autopsy case Human blood and urine Fatal intoxication Gas chromatography–mass spectrometry 

Notes

Compliance with ethical standards

Conflict of interest

There are no financial or other relations that could lead to conflicts of interest.

Ethical approval

The analyses of toxic substances were requested by judicial authorities with documentation. The blank blood and urine (about 10 mL, respectively) were collected from a healthy volunteer with informed consent.

References

  1. 1.
    Hahn T, Avella J, Lehrer M (2006) A motor vehicle accident fatality involving the inhalation of 1,1-difluoroethane. J Anal Toxicol 30:638–642CrossRefGoogle Scholar
  2. 2.
    Xiong Z, Avella J, Wetli CV (2004) Sudden death caused by 1,1-difluoroethane inhalation. J Forensic Sci 49:627–629CrossRefGoogle Scholar
  3. 3.
    Avella J, Wilson JC, Lehrer M (2006) Fatal cardiac arrhythmia after repeated exposure to 1,1-difluoroethane (DFE). Am J Forensic Med Pathol 27:58–60CrossRefGoogle Scholar
  4. 4.
    Jufer RA, Callery RT (2003) A case of fatal difluoroethane intoxication (abstract no. K31). Proceedings of the American Academy of Forensic Sciences, Chicago IL Annual Meeting IX: 318–319. https://www.aafs.org/up-content/uploads/ProceedingsChicago2003.pdf. Accessed Mar 2018
  5. 5.
    Shepherd RT (1989) Mechanism of sudden death associated with volatile substance abuse. Hum Toxicol 8:287–297CrossRefGoogle Scholar
  6. 6.
    Sakai K, Maruyama-Maebashi K, Takatsu A, Fukui K, Nagai T, Aoyagi M, Ochiai E, Iwadate K (2011) Sudden death involving inhalation of 1,1-difluoroethane (HFC-152a) with spray cleaner: three case reports. Forensic Sci Int 206:e58–e61CrossRefGoogle Scholar
  7. 7.
    Sasaki C, Shinozuka T, Irie W, Nakamae T, Murakami C, Nakamaru N, Furukawa M, Nakamura S, Kurihara K (2009) A fatality due to inhalation of 1,1-difluoroethane (HFC-152a) with a peculiar device. Forensic Toxicol 27:45–48CrossRefGoogle Scholar
  8. 8.
    Causon R (1997) Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion. J Chromatogr B 689:175–180CrossRefGoogle Scholar
  9. 9.
    Saka K, Uemura K, Shintani-Ishida K, Yoshida K (2008) Determination of amobarbital and phenobarbital in serum by gas chromatography-mass spectrometry with addition of formic acid to the solvent. J Chromatogr B 869:9–15CrossRefGoogle Scholar
  10. 10.
    US Department of Health and Human Services, Food and Drug Administration (FDA), Center for Drug Evaluation and Research (CDER), Center for Vaterinary Medicine (CVM) (2018) Bioanalytical method validation: guidance for industry. https://www.fda.gov/downloads/drugs/guidances/ucm070107.Pdf. Accessed May 2018
  11. 11.
    Villas-Bôas SG, Mas S, Akesson M, Smedsgaard J, Nielsen J (2005) Mass spectrometry in metabolome analysis. Mass Spectrom Rev 24:613–646CrossRefGoogle Scholar
  12. 12.
    Demeestere K, Dewulf J, Roo KD, Wispelaere PD, Langenhove HV (2008) Quality control in quantification of volatile organic compounds analysed by thermal desorption-gas chromatographymass spectrometry. J Chromatogr A 1186:348–357CrossRefGoogle Scholar
  13. 13.
    Avella J, Lehrer M, Zito SW (2008) A validated method for the quantitation of 1,1-difluoroethane using a gas in equilibrium method of calibration. J Anal Toxicol 32:680–687CrossRefGoogle Scholar
  14. 14.
    Vance C, Swalwell C, McIntyre IM (2012) Deaths involving 1,1-difluoroethane at the San Diego County Medical Examiner’s Office. J Anal Toxicol 36:626–633CrossRefGoogle Scholar
  15. 15.
    Harris D (2006) Volatile substance abuse. Arch Dis Child Educ Pract Ed 91:ep93–ep100CrossRefGoogle Scholar
  16. 16.
    Woollen BH, Marsh JR, Mahler JD, Auton TR, Makepeace D, Cocker I, Blain PG (1992) Human inhalation pharmacokinetics of chlorodifluoromethane (HCFC22). Int Arch Occup Environ Health 64:383–387CrossRefGoogle Scholar
  17. 17.
    Broussard LA, Brustowicz T, Pittman T, Atkins KD, Presley L (1997) Two traffic related fatalities related to the use of difluoroethane. J Forensic Sci 42:1186–1187CrossRefGoogle Scholar
  18. 18.
    Iseki K, Tomonaga A, Hayashida A, Seino K, Shinozaki K, Haneda T, Yamazaki K (2012) Fatal suffocation following inhalation of 1,1-difluoroethane (DFE) (in Japanese with English abstract). J Jpn Assoc Acute Med 23:775–780Google Scholar

Copyright information

© Japanese Association of Forensic Toxicology and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Suguru Torimitsu
    • 1
    • 2
  • Yusuke Fujii
    • 1
  • Kanju Saka
    • 1
  • Hiroko Abe
    • 2
  • Yohsuke Makino
    • 1
    • 2
  • Fumiko Chiba
    • 1
    • 2
  • Hirotaro Iwase
    • 1
    • 2
  1. 1.Department of Forensic Medicine, Graduate School of MedicineThe University of TokyoTokyoJapan
  2. 2.Department of Legal Medicine, Graduate School of MedicineChiba UniversityChibaJapan

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