Simultaneous determination of tryptamine analogues in designer drugs using gas chromatography–mass spectrometry and liquid chromatography–tandem mass spectrometry
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In recent years, a large number of tryptamine-based designer drugs have been encountered in forensic samples. We have developed simultaneous analytical methods for 14 tryptamine analogues using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–tandem mass spectrometry (LC–MS–MS). Trimethylsilyl (TMS) derivatives of the analytes were separated on a DB-1ms column within 15 min. The structural isomers could be differentiated by electron ionization GC–MS. LC–MS–MS with a C18 column could separate structural isomers of tryptamines except for a combination of 5-methoxy-N,N-diethyltryptamine and 5-methoxy-N-methyl-N-isopropyltryptamine. Higher collision energy gave different product ion spectra between the structural isomers. The results indicate that GC–MS is the first choice for identification of tryptamines, preferably after TMS derivatization, and LC–MS–MS can be used as a complementary approach for the unequivocal differentiation of tryptamine isomers.
KeywordsTryptamine analogues Designer drug Structural isomer GC–MS LC–MS–MS
This work was supported in part by the R&D Program for Implementation of Anti-Crime and Anti-Terrorism Technologies for a Safe and Secure Society, Funds for Integrated Promotion of Social System Reform and Research and Development of the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
Conflict of interest
There are no financial or other relations that could lead to a conflict of interest.
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