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Forensic Toxicology

, Volume 32, Issue 1, pp 154–161 | Cite as

Simultaneous determination of tryptamine analogues in designer drugs using gas chromatography–mass spectrometry and liquid chromatography–tandem mass spectrometry

  • Yukiko Nakazono
  • Kenji Tsujikawa
  • Kenji Kuwayama
  • Tatsuyuki Kanamori
  • Yuko T. Iwata
  • Kazuna Miyamoto
  • Fumiyo Kasuya
  • Hiroyuki Inoue
Short Communication

Abstract

In recent years, a large number of tryptamine-based designer drugs have been encountered in forensic samples. We have developed simultaneous analytical methods for 14 tryptamine analogues using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–tandem mass spectrometry (LC–MS–MS). Trimethylsilyl (TMS) derivatives of the analytes were separated on a DB-1ms column within 15 min. The structural isomers could be differentiated by electron ionization GC–MS. LC–MS–MS with a C18 column could separate structural isomers of tryptamines except for a combination of 5-methoxy-N,N-diethyltryptamine and 5-methoxy-N-methyl-N-isopropyltryptamine. Higher collision energy gave different product ion spectra between the structural isomers. The results indicate that GC–MS is the first choice for identification of tryptamines, preferably after TMS derivatization, and LC–MS–MS can be used as a complementary approach for the unequivocal differentiation of tryptamine isomers.

Keywords

Tryptamine analogues Designer drug Structural isomer GC–MS LC–MS–MS 

Notes

Acknowledgments

This work was supported in part by the R&D Program for Implementation of Anti-Crime and Anti-Terrorism Technologies for a Safe and Secure Society, Funds for Integrated Promotion of Social System Reform and Research and Development of the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

Conflict of interest

There are no financial or other relations that could lead to a conflict of interest.

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Copyright information

© Japanese Association of Forensic Toxicology and Springer Japan 2013

Authors and Affiliations

  • Yukiko Nakazono
    • 1
    • 2
  • Kenji Tsujikawa
    • 1
  • Kenji Kuwayama
    • 1
  • Tatsuyuki Kanamori
    • 1
  • Yuko T. Iwata
    • 1
  • Kazuna Miyamoto
    • 2
    • 3
  • Fumiyo Kasuya
    • 3
  • Hiroyuki Inoue
    • 1
  1. 1.National Research Institute of Police ScienceKashiwaJapan
  2. 2.Hitec, Inc.ChibaJapan
  3. 3.Biochemical Toxicology Laboratory, Faculty of Pharmaceutical SciencesKobegakuin UniversityKobeJapan

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