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Forensic Toxicology

, 26:23 | Cite as

Determination of fluoride in human whole blood and urine by gas chromatography-mass spectrometry

  • Shigetoshi Kage
  • Keiko Kudo
  • Naoki Nishida
  • Hideaki Ikeda
  • Naofumi Yoshioka
  • Noriaki IkedaEmail author
Short Communication

Abstract

We developed a simple and sensitive method for determination of fluoride in human whole blood and urine using gas chromatography-mass spectrometry (GC-MS). Fluoride was alkylated with pentafluorobenzyl bromide in a mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed by GC-MS in the positive-ion electron-impact mode. The lower limit of detection for the compound was 0.5 mg/l for both matrices. The calibration curve for fluoride was linear over the concentration range of 1–100 mg/l. The precision and accuracy of the method were evaluated, and relative standard deviation was within 10%. Using this method, levels of fluoride in whole blood and urine were determined in a case of poisoning caused by hydrofluoric acid exposure.

Keywords

Fluoride Hydrofluoric acid Gas poisoning GC-MS Pentafluorobenzyl Whole blood 

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Copyright information

© Japanese Association of Forensic Toxicology 2008

Authors and Affiliations

  • Shigetoshi Kage
    • 1
  • Keiko Kudo
    • 2
  • Naoki Nishida
    • 3
  • Hideaki Ikeda
    • 1
  • Naofumi Yoshioka
    • 4
  • Noriaki Ikeda
    • 2
    Email author
  1. 1.Forensic Science LaboratoryFukuoka Prefectural Police HeadquartersFukuokaJapan
  2. 2.Department of Forensic Pathology and Sciences, Graduate School of Medical SciencesKyushu UniversityHigashi-ku, FukuokaJapan
  3. 3.Department of Legal Medicine, Graduate School of Medicine and Pharmaceutical SciencesUniversity of ToyamaToyamaJapan
  4. 4.Division of Forensic Sciences, Department of Social MedicineAkita University School of MedicineAkitaJapan

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