Forensic Toxicology

, Volume 26, Issue 1, pp 6–12 | Cite as

Simultaneous analysis of six phenethylamine-type designer drugs by TLC, LC-MS, and GC-MS

  • Kohei Kanai
  • Kenichi Takekawa
  • Takuya Kumamoto
  • Tsutomu Ishikawa
  • Takeshi OhmoriEmail author
Original Article


Extensive data are presented for simultaneous analysis of six phenethylamine-type designer drugs. The drugs are 2,5-dimethoxyphenethylamine (2C-H), 2,5-dimethoxyamphetamine (2,5-DMA), 4-bromo-2,5-dimethoxyphenethylamine (2C-B), 4-bromo-2,5-dimethoxyamphetamine (DOB), 4-iodo-2,5-dimethoxyphenethylamine (2C-I), and 4-iodo-2,5-dimethoxyamphetamine (DOI). The data include their proton nuclear magnetic resonance (NMR) spectra, infrared spectra, retention times detected by liquid chromatography (LC) and gas chromatography (GC), thin-layer chromatography (TLC) data using seven solvent mixture systems, electrospray ionization (ESI) mass spectra without derivatization, and electron ionization (EI) mass spectra with trifl uoroacetyl (TFA) derivatization. Quantitative reliability was also tested for these compounds by LC-mass spectrometry (MS) and GC-MS in high concentration ranges. The data concerned is intended to serve for screening, identification, and quantitation of the drugs contained in seized tablets, crystals, and powder; the data are very useful for such purposes in actual forensic toxicological practice.


2,5-Dimethoxyphenethylamine analogues Designer drugs 2C-B DOB 2C-I DOI 


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  1. 1.
    Jacob P III, Shulgin AT (1994) Structure-activity relationships of the classic hallucinogens and their analogs. In: Lin GC, Glennon RA (eds) Hallucinogens: an update, NIDA Research Monograph vol 146. NIDA, Rockville, pp 74–91Google Scholar
  2. 2.
    Delliou D (1983) 4-Bromo-2,5-dimethoxyamphetamine: psychoactivity, toxic effects and analytical methods. Forensic Sci Int 21:259–267PubMedCrossRefGoogle Scholar
  3. 3.
    Glennon RA, Titeler M, Lyon RA (1998) A preliminary investigation of the psychoactive agent 4-bromo-2,5-dimethoxyphenethylamine: a potential drug of abuse. Pharmacol Biochem Behav 30:597–601CrossRefGoogle Scholar
  4. 4.
    Shulgin AT, Carter MF (1975) Centrally active phenethylamines. Psychopharmacol Commun 1:93–98PubMedGoogle Scholar
  5. 5.
    Ragan FA, Hite SA, Samuels MS, Garey RE (1985) 4-Bromo-2,5-dimethoxyphenethylamine: identification of a new street drug. J Anal Toxicol 9:91–93PubMedGoogle Scholar
  6. 6.
    Giroud C, Augsburger M, Rivier L, Mangin P (1998) 2C-B: a new psychoactive phenylethylamine recently discovered in ecstasy tablets sold on the Swiss black market. J Anal Toxicol 22:345–354PubMedGoogle Scholar
  7. 7.
    De Boer D, Gijzels DJ, Bosman IJ, Maes RAA (1999) More data about new psychoactive drug 2C-B. J Anal Toxicol 23: 227–228PubMedGoogle Scholar
  8. 8.
    Theobald DS, Putz M, Schneider E, Maurer HH (2006) New designer drug 4-iodo-2,5-dimethoxy-beta-phenethylamine (2C-I): studies on its metabolism and toxicological detection in rat urine using gas chromatographic/mass spectrometric and capillary electrophoretic/mass spectrometric techniques. J Mass Spectrom 41:872–886PubMedCrossRefGoogle Scholar
  9. 9.
    Shulgin AT, Shulgin A (1992) Pihkal, a chemical love story. Transform, BerkeleyGoogle Scholar
  10. 10.
    Pharmaceutical Society of Japan (ed) (2006) Standard methods of chemical analysis in poisoning-with commentary (in Japanese). Tokyo Kagaku Dojin, TokyoGoogle Scholar
  11. 11.
    Theobald DS, Susanna F, Maurer HH (2005) New designer drug, 2,5-dimethoxy-4-propylthio-β-phenethylamine (2C-T-7): studies on its metabolism and toxicological detection in rat urine using gas chromatography/mass spectrometry. J Mass Spectrom 40:105–116PubMedCrossRefGoogle Scholar

Copyright information

© Japanese Association of Forensic Toxicology 2008

Authors and Affiliations

  • Kohei Kanai
    • 1
  • Kenichi Takekawa
    • 1
  • Takuya Kumamoto
    • 2
  • Tsutomu Ishikawa
    • 2
  • Takeshi Ohmori
    • 3
    Email author
  1. 1.Forensic Science LaboratoryYamanashi Prefectural Police H.Q.YamanashiJapan
  2. 2.Graduate School of Pharmaceutical ScienceChiba UniversityChibaJapan
  3. 3.National Research Institute of Police ScienceKashiwa, ChibaJapan

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