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Forensic Toxicology

, Volume 24, Issue 1, pp 2–7 | Cite as

Two simple methods for enantiomeric analyses of urinary amphetamines by GC/MS using deuterium-labeled l-amphetamines as internal standards

  • Zhao Liu
  • Kenji Hara
  • Seiichi Kashimura
  • Junting Liu
  • Hiroshi Fujii
  • Masayuki Kashiwagi
  • Aya Miyoshi
  • Tomoko Yanai
  • Mitsuyoshi Kageura
Original Article

Abstract

Two simple methods for enantiomeric analyses of amphetamines in urine by gas chromatography-mass spectrometry (GC-MS) using l-amphetamine-d 3 and l-methamphetamine-d 6 as internal standards are presented. One method (method A) employs extractive derivatization on a diatomaceous column with (S)-(-)-N-(trifluoroacetyl)prolyl chloride (TPC) followed by separation with a conventional capillary column. The second method (method B) uses headspace solid-phase microextraction (HD-SPME) after derivatization with heptafluoro-n-butyryl chloride (HFB), followed by separation with an enantiomeric capillary GC column. By the two methods, all enantiomers were well separated in each chromatogram, and good linearity was obtained in practical concentration ranges (0.1–1.6μg/ml for method A and 0.05–1μg/ml for method B) for every compound by selected-ion monitoring. The precision studies indicated satisfactory coefficients of variation (<5%) for every enantiomer at 0.1μg/ml by both methods. Both methods were also evaluated by applying them to an actual poisoning case. Both methods are recommended for use in forensic analysis, because of their simplicity, high precision, and sufficient sensitivity.

Keywords

Amphetamine Methamphetamine Enantiomeric analysis GC-MS Trifluoroacetylprolyl derivatization 

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Copyright information

© Japanese Association of Forensic Toxicology and Springer-Verlag Tokyo 2006

Authors and Affiliations

  • Zhao Liu
    • 1
  • Kenji Hara
    • 1
  • Seiichi Kashimura
    • 1
  • Junting Liu
    • 1
    • 2
  • Hiroshi Fujii
    • 1
  • Masayuki Kashiwagi
    • 1
  • Aya Miyoshi
    • 1
  • Tomoko Yanai
    • 1
  • Mitsuyoshi Kageura
    • 1
  1. 1.Department of Forensic MedicineFukuoka University School of MedicineFukuokaJapan
  2. 2.Department of Forensic ChemistryChina Medical UniversityShenyangP.R. China

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