Isolation, structure elucidation, tyrosinase inhibitory, and antioxidant evaluation of the constituents from Angelica dahurica roots
- 136 Downloads
Two undescribed phenolic compounds, angelicols A (1) and B (2) and one undescribed coumarin rhamnoside, angelicoside A (3), together with 17 known compounds (4–20) were isolated from the roots of Angelica dahurica. Their structures were characterized by physical data analyses such as NMR, HRESIMS, and X-ray diffraction. Compounds 2, 3, 5, 6 and L-ascorbic acid (positive control) exhibited obvious DPPH radical scavenging activities with IC50 values of 0.36 mM, 0.43 mM, 0.39 mM, 0.44 mM, 0.25 mM, respectively. At a concentration of 25 μM, all compounds showed weaker tyrosinase inhibition activities (%inhibition < 5%) than kojic acid (26.00 ± 0.67%, IC50 = 44.29 ± 0.06 μM).
KeywordsAngelica dahurica Tyrosinase X-ray diffraction Antioxidant
We are grateful to Lanxiang Dai for the collection of Angelica dahurica roots and Prof. Lin Yang at Lanzhou University of Technology for the authentication of the plant material. This work was financially supported by National Natural Science Foundation of China (no. 21702178), Key Scientific Research Program in Universities of Henan Province (no. 18A350010), Undergraduate Training Program for Innovation and Entrepreneurship of Henan Province (nos. 201910480011, 201910480017), Science and Technology Project of Henan Province (no. 182102311108), and Excellent Young Key Teacher Funding Project of Xuchang University (no. 2017).
- 1.Chinese Pharmacopoeia Commission (2015) Pharmacopoeia of the People’s Republic of China, vol I. Chemical Industry Press, Beijing, pp 105–106Google Scholar
- 8.Xie Q, Shun-Xiang L, Duan-Fang L, Wang W, Tekwani B, Hui-Yong H, Abbas ur Rehman AJ, Schrader KK, Duke SO (2016) Bio-pesticidal and antimicrobial coumarins from Angelica dahurica (Fisch. Ex Hoffm). Rec Nat Prod 10:294–306Google Scholar
- 29.Zhao AH, Yang XB, Yang XW, Zhang YB, Xu W, Liu JX (2012) Biotransformation products of phellopterin by rat liver microsomes and the inhibition on NO production in LPS-activated RAW264.7 cells. J Asian Nat Prod Res 20:697–707Google Scholar