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Four new cucurbitane-type triterpenes from Momordica charantia L. with their cytotoxic activities and protective effects on H2O2-damaged pancreatic cells

  • Jiayin Yue
  • Yuanyuan Sun
  • Jing Xu
  • Xiaoshu ZhangEmail author
  • Yuqing ZhaoEmail author
Original Paper
  • 215 Downloads

Abstract

Four new cucurbitane-type triterpenes were isolated from the fruit of Momordica charantia L. The structures of the new compounds were identified based on HR-ESI-MS and 1D- and 2D-NMR spectroscopic methods. The cytotoxicity of the isolated compounds was evaluated using three human cancer cell lines, HeLa, Caco2, and U87. Compound 3 exhibited significant cytotoxic activity against HeLa cells with an IC50 value of 11.18 μM. Additionally, the cytoprotective activity of these compounds was determined in vitro against H2O2-induced pancreatic injury. The results revealed that all the compounds obtained possess cytoprotective effects against H2O2-induced injury in MIN6 β-cells at a concentration of 10 μM.

Keywords

Momordica charantia L. Cucurbitane-type triterpenoid sapogenins Cytotoxic activity Protection of pancreatic cell oxidative injury 

Notes

Acknowledgements

We are glad to acknowledge the Analytical Center of Shenyang Pharmaceutical University for NMR measurements. The research was supported by National Nature Science Foundation of China (Grant No. 81703389), Youth development support plan of Shenyang Pharmaceutical University (ZQN2016023), Liaoning E & T research center for industrial chromatographic preparation of Natural Products (No. 2008402021), Technology Platform of Industrialization Chromatographic Preparation for Standard Extract of Traditional Chinese Medicine (2010ZX09401-304), and Liaoning (FGW) Engineering Technology Research Center for industrial chromatographic preparation of natural innovative drugs materials (2017-1007).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

11418_2019_1336_MOESM1_ESM.docx (2.8 mb)
Supplementary material 1 (DOCX 2894 kb)

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Copyright information

© The Japanese Society of Pharmacognosy 2019

Authors and Affiliations

  1. 1.The Precise Medicine Center, Key Laboratory of Microbiology College of Basic MedicineShenyang Medical CollegeShenyangPeople’s Republic of China
  2. 2.School of Functional Food and WineShenyang Pharmaceutical UniversityShenyangPeople’s Republic of China
  3. 3.Key Laboratory of Structure-Based Drug Design and Discovery Ministry of EducationShenyang Pharmaceutical UniversityShenyangChina
  4. 4.School of Life Science and BiopharmaceuticsShenyang Pharmaceutical UniversityShenyangPeople’s Republic of China

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