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Ecological, phylogenetical, and pharmacognostical characteristics of Aconitum kiyomiense endemic to Hida highlands, Takayama city, Gifu Prefecture, Japan

  • Motoyasu MinamiEmail author
  • Misaki Yasueda
  • Tomoyasu Shirako
  • Tetuo Murakami
  • Takako Mori
  • Taichi Fujii
  • Toshiyuki Atsumi
  • Toshiro Shibata
  • Yuichi Kadota
Original Paper
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Abstract

Aconitum kiyomiense Kadota (Ranunculaceae) is endemic to Takayama city, Gifu Prefecture, central Japan. We collected specimens from marshes and flood plains at altitudes ranging from 852 to 1085 m and from a new habitat consisting of a mesic meadow in the subalpine belt (1681 m). Glabrous pedicels and flowering sequence of inflorescence were used for identification, but intra-species variations in the pilus of pedicels (glabrous, pilose, and chimeric types) were observed. Although the flowering sequence has been reported as both indeterminate and determinate, all specimens in the present study were determinate. No intra-species variation was detected via partial nuclear internal transcribed space, and sequences did not match another 17 East Eurasian continent subgenus Aconitum species. The chloroplast trnL–trnF intergenic spacer region (trnL–trnF) showed three different haplotypes. The trnL–trnF dominant haplotype sequence was identical to that of A. kusnezoffii growing on the Eurasian continent, suggesting that A. kiyomiense is more primitive than other Japanese aconitum and a relic species of the Eurasian continent. We report the first detection of aconitine alkaloids in the tuberous roots, which exhibited aconitine alkaloid contents varying from 0.32 to 4.05 mg/g dry weight (mg/g) for aconitine, 0.02 to 4.12 mg/g for hypaconitine, undetectable to 0.05 mg/g for jesaconitine, and 0.42 to 3.76 mg/g for mesaconitine. The variation of aconitine alkaloid components and contents appeared to be random and did not vary with inflorescence phenotype, trnL–trnF haplotype, environmental habitat conditions, or the geographic region of the collection sites. Since most populations showed no genetic intra-variation, it will be necessary to maintain the continuity of habitats and designate areas for conservation of genetic diversity at the population level.

Keywords

Aconitum kiyomiense Aconitine alkaloids trnL–trnF intergenic spacer region ITS 

Notes

Acknowledgements

The authors thank a Guest professor Kazuyuki Morise of Chubu University and the Takayama city government for support and Ms. Mai Okada and Mr. Ryoji Kuno of Chubu University for providing water temperature data of NAT-2 site. This work was supported by the Challenge Site of Chubu University (No. sprt155).

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Copyright information

© The Japanese Society of Pharmacognosy 2019

Authors and Affiliations

  • Motoyasu Minami
    • 1
    Email author
  • Misaki Yasueda
    • 1
  • Tomoyasu Shirako
    • 1
  • Tetuo Murakami
    • 1
  • Takako Mori
    • 1
  • Taichi Fujii
    • 1
  • Toshiyuki Atsumi
    • 2
  • Toshiro Shibata
    • 3
  • Yuichi Kadota
    • 4
  1. 1.College of Bioscience and BiotechnologyChubu UniversityKasugaiJapan
  2. 2.Department of Pharmaceutical Sciences, School of Pharmaceutical SciencesKyushu University of Health and WelfareNobeokaJapan
  3. 3.Research Center for Medicinal Plant ResourcesNational Institutes of Biomedical Innovation, Health and NutritionTsukubaJapan
  4. 4.Department of BotanyNational Museum of Nature and ScienceTsukubaJapan

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