Flavonoids isolated from the flowers of Pulsatilla flavescens and their anti-piroplasm activity
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Pulsatilla species are known as “Yargui”, and their flowers are traditionally used in Mongolia as a tonic and for the treatment of inflammatory diseases. By chemical investigation of P. flavescens flowers, 21 flavonoids, including a new chalcone C-glucoside, chalconaringenin 2′-O-β-d-glucopyranosyl-5′-β-d-glucopyranoside, and two new flavanone C-glucosides, (2R)- and (2S)-naringenin 8-β-d-glucopyranosyl-4′-O-β-d-glucopyranoside, were isolated. The absolute configurations of the seven flavanone glucosides were elucidated by ECD spectra. For the isolated compounds, inhibitory activity against Babesia caballi and Theileria equi, which cause fatal diseases in horses, was estimated. Although most of the isolated chalcone and flavanone derivatives did not show any anti-piroplasm activity, all the isolated flavone and flavonol derivatives showed moderate effects against B. caballi and/or T. equi.
KeywordsPulsatilla flavescens Yargui Flavonoids Anti-piroplasma activity
We thank Dr. B. Odonbayar, Mr. T. Ishikawa, and Mr. Y. Muraki, Mr. S. Sato, and Mr. T. Matsuki, Tohoku Medical and Pharmaceutical University, for assistance with the compound isolations and the MS measurements. This work was supported by JICA M-JEED project, Grant-in-aid project from Mongolian Foundation of Science and Technology (2018/58), and Grant for Advanced Research at National University of Mongolia (P2018-3601). This work was partially supported by the Kanno Foundation of Japan, a Cooperative Research Grant (29-joint-6, 30-joint-11) from the National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, and AMED/JICA SATREPS.
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Conflicts of interest
The authors indicate that there is no conflict of interest.
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