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Flavonoids isolated from the flowers of Pulsatilla flavescens and their anti-piroplasm activity

  • Dorj Ganchimeg
  • Badarch Batbold
  • Toshihiro MurataEmail author
  • Bekh-Ochir Davaapurev
  • Tserendorj Munkhjargal
  • Bumduuren Tuvshintulga
  • Keisuke Suganuma
  • Ikuo Igarashi
  • Buyanmandakh Buyankhishig
  • Kenroh Sasaki
  • Dulamjav Batsuren
  • Javzan Batkhuu
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Abstract

Pulsatilla species are known as “Yargui”, and their flowers are traditionally used in Mongolia as a tonic and for the treatment of inflammatory diseases. By chemical investigation of P. flavescens flowers, 21 flavonoids, including a new chalcone C-glucoside, chalconaringenin 2′-O-β-d-glucopyranosyl-5′-β-d-glucopyranoside, and two new flavanone C-glucosides, (2R)- and (2S)-naringenin 8-β-d-glucopyranosyl-4′-O-β-d-glucopyranoside, were isolated. The absolute configurations of the seven flavanone glucosides were elucidated by ECD spectra. For the isolated compounds, inhibitory activity against Babesia caballi and Theileria equi, which cause fatal diseases in horses, was estimated. Although most of the isolated chalcone and flavanone derivatives did not show any anti-piroplasm activity, all the isolated flavone and flavonol derivatives showed moderate effects against B. caballi and/or T. equi.

Keywords

Pulsatilla flavescens Yargui Flavonoids Anti-piroplasma activity 

Notes

Acknowledgments

We thank Dr. B. Odonbayar, Mr. T. Ishikawa, and Mr. Y. Muraki, Mr. S. Sato, and Mr. T. Matsuki, Tohoku Medical and Pharmaceutical University, for assistance with the compound isolations and the MS measurements. This work was supported by JICA M-JEED project, Grant-in-aid project from Mongolian Foundation of Science and Technology (2018/58), and Grant for Advanced Research at National University of Mongolia (P2018-3601). This work was partially supported by the Kanno Foundation of Japan, a Cooperative Research Grant (29-joint-6, 30-joint-11) from the National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, and AMED/JICA SATREPS.

Compliance with ethical standards

Conflicts of interest

The authors indicate that there is no conflict of interest.

Supplementary material

11418_2019_1294_MOESM1_ESM.pdf (878 kb)
Supplementary material 1 (PDF 878 kb)

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Copyright information

© The Japanese Society of Pharmacognosy 2019

Authors and Affiliations

  • Dorj Ganchimeg
    • 1
    • 2
  • Badarch Batbold
    • 1
  • Toshihiro Murata
    • 3
    Email author
  • Bekh-Ochir Davaapurev
    • 1
  • Tserendorj Munkhjargal
    • 4
    • 5
  • Bumduuren Tuvshintulga
    • 4
  • Keisuke Suganuma
    • 4
    • 6
  • Ikuo Igarashi
    • 4
  • Buyanmandakh Buyankhishig
    • 3
  • Kenroh Sasaki
    • 3
  • Dulamjav Batsuren
    • 7
  • Javzan Batkhuu
    • 1
  1. 1.School of Engineering and Applied SciencesNational University of MongoliaUlaanbaatarMongolia
  2. 2.School of Natural Science and TechnologyKhovd UniversityKhovdMongolia
  3. 3.Department of PharmacognosyTohoku Medical and Pharmaceutical UniversityAoba-ku, SendaiJapan
  4. 4.National Research Center of Protozoan DiseasesObihiro University of Agriculture and Veterinary MedicineInada, ObihiroJapan
  5. 5.Institute of Veterinary MedicineMongolian University of Life ScienceZaisan, UlaanbaatarMongolia
  6. 6.Research Center for Global AgromedicineObihiro University of Agriculture and Veterinary MedicineInada, ObihiroJapan
  7. 7.Institute of Chemistry and Chemical TechnologyMongolian Academy of ScienceUlaanbaatarMongolia

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