Mutagenetic and anti-allergic studies for evaluation of extracts of Coptis Rhizome produced by an artificial hydroponic system
- 47 Downloads
As a part of the investigation of the safety and efficacy of the cultivated Coptis japonica rhizome extracts using an artificial hydroponic cultivation system, the mutagenetic and anti-allergic activities were evaluated. Some extracts of commercial crude drugs of Coptis sp. were also evaluated for the comparison. None of the extracts showed a significant mutagenicity in Salmonella typhimurium TA102 by the Ames tests, but all the extracts showed in S. typhimurium TA98. The extracts of the hydroponically cultivated rhizomes showed anti-allergic activities against contact hypersensitivity as well as those of commercial crude drugs of Coptis sp. These results suggested the potential of the hydroponically cultivated rhizomes as one of the alternative sources for the medicinal usage.
KeywordsCoptis japonica Berberine Hydroponic cultivation
This study was supported by a Health and Labour Sciences Research Grant from the Ministry of Health, Labour and Welfare of Japan, a Grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The authors would like to thank the Japan Kampo Medicines Manufacturers Association and Tokyo Crude Drug Association for providing the commercial Coptis crude drugs.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- 1.The Ministry of Health, Labour and Welfare of Japan (2016) The Japanese pharmacopoeia, 17th edn. The Ministry of Health, Labour and Welfare of Japan, TokyoGoogle Scholar
- 2.Japan Kampo Medicines Manufacturers Association (2016) Report on investigation of usage of the crude drugs for Kampo preparation–the usage in FY2013 and FY2014. http://www.nikkankyo.org/serv/pdf/shiyouryou-chousa04.pdf. Accessed 6 July 2018
- 4.Minami M, Yomo T, Hasegawa C, Ohe C, Ashida K, Sugino M (1995) Production of medicinal plants by soilless culture system. I. Studies of morphological characteristics and saikosaponins content in Bupleurum falcatum cultivated by Ebb & Flood system. Yakugaku Zasshi 115:832–842CrossRefPubMedGoogle Scholar
- 5.Kakutani K, Ozaki K, Watababe H, Tomoda K (1997) Preparation of licorice seedling by node culture, and glycyrrhizin production by several nutricultures using the seedling. Shoyakugaku Zasshi 51:447–451Google Scholar
- 6.Kakutani K (2003) Glycyrrhizin production of licorice by nutricultures using several material. Bull Pharm Res Technol Inst 12:133–138Google Scholar
- 7.Yoshimatsu K (2010) A cultivation apparatus and a cultivation method. Japan Kokai Tokkyo Koho JP 2010-273648 (Patent number)Google Scholar
- 8.Sawada H, Saito T, Kudo T, Ono T, Yoshimatsu K (2012) A hydroponics system and the hydroponics method. Japan Kokai Tokkyo Koho JP 2012-100573 (Patent number)Google Scholar
- 9.Yoshimatsu K (2012) Innovative cultivation: hydroponics of medicinal plants in the closed-type cultivation facilities. J Tradit Med 29:30–34Google Scholar
- 10.Yoshimatsu K, Kawano N, Inui T (2012) Selection and growing of Glycyrrhiza uralensis suitable for licorice production in plant factory. BRAIN Techno News 149:20–28Google Scholar
- 11.Inui T, Ikeda M, Takagi M, Kawano N, Kawahara N, Yoshimatsu K, Yoshimatsu K (2017) Aiming for improvement in productivity of Coptis japonica. Agric Biotechnol 1:757–759Google Scholar
- 12.Akiyama H, Nose M, Ohtsuki N, Hisaka S, Takiguchi H, Tada A, Sugimoto N, Fuchino H, Inui T, Kawano N, Hayashi S, Hishida A, Kudo T, Sugiyama K, Abe Y, Mutsuga M, Kawahara Yoshimatsu K (2017) Evaluation of the safety and efficacy of Glycyrrhiza uralensis root extracts produced using artificial hydroponic and artificial hydroponic-field hybrid cultivation systems. J Nat Med 71:265–271CrossRefPubMedGoogle Scholar
- 14.Inui T, Kawano N, Shitan N, Yazaki K, Kiuchi F, Kawahara N, Sato F, Yoshimatsu K (2012) Improvement of benzylisoquinoline alkaloid productivity by overexpression of 3′-hydroxy-N-methylcoclaurine 4′-O-methyltransgerase in transgenic Copits japonica plants. Biol Pharm Bull 35:650–659CrossRefPubMedGoogle Scholar
- 15.Kawano N, Inui T, Yoshimatsu K, Hiyama H (2017) The hydroponics method and selection method of Coptis japonica. Japan Kokai Tokkyo Koho JP 2017-045217 (Patent number)Google Scholar
- 20.Mo C, Wang L, Zhang J, Numazawa S, Tang H, Tang X, Han X, Li J, Yang M, Wang Z, Wei D, Xiao H (2014) The crosstalk between Nrf2 and AMPK signal pathways is important for the anti-inflammatory effect of berberine in LPS-stimulated macrophages and endotoxin-shocked mice. Antioxid Redox Signal 20:574–588. https://doi.org/10.1089/ars.2012.5116 CrossRefPubMedPubMedCentralGoogle Scholar