Luteolin decreases the yield of influenza A virus in vitro by interfering with the coat protein I complex expression

  • Haiyan Yan
  • Linlin Ma
  • Huiqiang Wang
  • Shuo Wu
  • Hua Huang
  • Zhengyi Gu
  • Jiandong Jiang
  • Yuhuan LiEmail author
Original Paper


Influenza is an acute transmissible respiratory infectious disease in humans and animals with high morbidity and mortality. It was reported that luteolin, extracted from Chinese herbs, could potently inhibit influenza virus replication in vitro. To assess the effect and explore the fundamental mechanism of luteolin, we infected several cell lines with two subtypes of influenza A virus (IAV), including A/Jiangxi/312/2006 (H3N2) and A/Fort Monmouth/1/1947 (H1N1) and demonstrated that luteolin suppressed the replication of IAV by cytopathic effect reduction method, qRT-PCR, immunofluorescence and Western blot assays. A time-of-addition assay indicated that this compound interfered with viral replication at the early stage of infection. In addition, we found that luteolin suppressed coat protein I complex expression, which was related to influenza virus entry and endocytic pathway. Overall, our findings demonstrated the antiviral effect of luteolin against IAV and its novel antiviral mechanism.


Influenza A virus Luteolin Antiviral activity COPI 



This work was supported by the National Nature Science Foundation of China(NSFC) (Grant number: 81621064 and U1303224); The National Science and Technology Major Projects for "Major New Drugs Innovation and Development" (2018ZX09711003) and CAMS Innovation Fund for Medical Sciences (CIFMS) (No. 2017-I2M-3-010).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© The Japanese Society of Pharmacognosy 2019

Authors and Affiliations

  • Haiyan Yan
    • 1
  • Linlin Ma
    • 2
  • Huiqiang Wang
    • 1
  • Shuo Wu
    • 1
  • Hua Huang
    • 3
  • Zhengyi Gu
    • 3
  • Jiandong Jiang
    • 1
  • Yuhuan Li
    • 1
    Email author
  1. 1.Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina
  2. 2.Key Laboratory of Molecular Imaging of Shanghai Education CommissionShanghai University of Medicine & Health SciencesShanghaiChina
  3. 3.Xinjiang Institute of Materia MedicaÜrümqiChina

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