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Anti-inflammatory activities of Ophiopogonis Radix on hydrogen peroxide-induced cellular senescence of normal human dermal fibroblasts

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Abstract

Ophiopogonis Radix (Ophiopogon root), which nourishes the yin, has been used in clinical practice to promote fluid secretion and to moisturize the lungs and skin in traditional Chinese and Japanese (Kampo) medicine. To evaluate this traditional medicinal effect, we investigated the anti-chronic inflammatory effect of Radix Ophiopogonis on senescent cells. Conversely, although several phenotypes of Ophiopogon japonicus Ker-Gawler (Liliaceae) are prevalent in Japan and China, we used these Ophiopogon roots by considering them as one crude drug, Ophiopogonis Radix. In this study, it was revealed that there are two chemotypes (Types A and B) in the root of the original plant, O. japonicus. Methylophiopogonanone A (compound 1) and methylophiopogonanone B (compound 2) were isolated as index compounds from Type A and compound 1 and ophiopogonanone A (compound 3) from Type B. In addition, ophiopogonin B (compound 4) was isolated as the main steroidal saponin from both Type A and B. The results indicated that two different methanol extracts (from Types A and B) and the main constituents of O. japonicus (compound 1–4), significantly downregulated the expression of interleukin (IL)-6 and IL-8, which were enhanced by senescent normal human dermal fibroblasts. Moreover, the two different methanol extracts and compounds 1–4 decreased IL-6 production in a strong and concentration-dependent manner by the ELISA method.

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Acknowledgements

The authors are grateful to Dr. K. Minoura for NMR spectra recordings and Dr. M. Fujitake for mass spectra recordings at the Osaka University of Pharmaceutical Sciences.

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Authors and Affiliations

  1. Department of Natural Products Research, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka, 569-1094, Japan

    Yumi Kitahiro, Mei Muto, Kazuo Ozaki & Makio Shibano

  2. Department of Biodefense and Regulation, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka, 569-1094, Japan

    Atsushi Koike

  3. Oppen Cosmetic Co., Ltd., 61-1 Yamadera-cho, Kusatsu, Shiga, 525-0042, Japan

    Aska Sonoki

Authors
  1. Yumi Kitahiro
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  2. Atsushi Koike
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  3. Aska Sonoki
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  4. Mei Muto
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  5. Kazuo Ozaki
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  6. Makio Shibano
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Correspondence to Makio Shibano.

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Kitahiro, Y., Koike, A., Sonoki, A. et al. Anti-inflammatory activities of Ophiopogonis Radix on hydrogen peroxide-induced cellular senescence of normal human dermal fibroblasts. J Nat Med 72, 905–914 (2018). https://doi.org/10.1007/s11418-018-1223-9

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  • DOI: https://doi.org/10.1007/s11418-018-1223-9

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