Inhibition of melanin production by anthracenone dimer glycosides isolated from Cassia auriculata seeds
The methanol extract of Cassia auriculata seeds was found to inhibit melanogenesis in B16 melanoma 4A5 cells under conditions of theophylline stimulation. Two new phlegmacin-type anthracenone dimer glycosides, auriculataosides A and B, were isolated from the active methanol fraction, and their inhibitory effects were observed in the concentration range of 0.03 to 0.3 μM. Inhibition of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 protein expression was observed, suggesting that the inhibition of these factors is part of the mechanism of action underlying melanogenesis inhibition.
KeywordsCassia auriculate Anthracenone dimer glycoside Melanogenesis inhibitor Mechanism of action
This work was partly supported by JSPS KAKENHI Grant Number 16K08312 (H.M.).
Compliance with ethical standards
Conflict of interest
M. Yoshikawa and H. Matsuda have been received research Grants from N.T.H Co., Ltd. All other authors declare that they have no conflicts of interest.
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