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Inhibition of melanin production by anthracenone dimer glycosides isolated from Cassia auriculata seeds

  • Weicheng Wang
  • Yi Zhang
  • Souichi Nakashima
  • Seikou Nakamura
  • Tao Wang
  • Masayuki Yoshikawa
  • Hisashi MatsudaEmail author
Original Paper

Abstract

The methanol extract of Cassia auriculata seeds was found to inhibit melanogenesis in B16 melanoma 4A5 cells under conditions of theophylline stimulation. Two new phlegmacin-type anthracenone dimer glycosides, auriculataosides A and B, were isolated from the active methanol fraction, and their inhibitory effects were observed in the concentration range of 0.03 to 0.3 μM. Inhibition of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 protein expression was observed, suggesting that the inhibition of these factors is part of the mechanism of action underlying melanogenesis inhibition.

Keywords

Cassia auriculate Anthracenone dimer glycoside Melanogenesis inhibitor Mechanism of action 

Notes

Acknowledgements

This work was partly supported by JSPS KAKENHI Grant Number 16K08312 (H.M.).

Compliance with ethical standards

Conflict of interest

M. Yoshikawa and H. Matsuda have been received research Grants from N.T.H Co., Ltd. All other authors declare that they have no conflicts of interest.

Supplementary material

11418_2018_1276_MOESM1_ESM.docx (532 kb)
Supplementary material 1 (DOCX 533 kb)

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Copyright information

© The Japanese Society of Pharmacognosy 2019

Authors and Affiliations

  1. 1.Kyoto Pharmaceutical UniversityKyotoJapan
  2. 2.Tianjin State Key Laboratory of Modern Chinese MedicineTianjin University of Traditional Chinese MedicineTianjinChina
  3. 3.N.T.H Co., Ltd.TokyoJapan

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