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Journal of Natural Medicines

, Volume 72, Issue 2, pp 399–408 | Cite as

Discrimination of Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus based on fingerprint profiles of hydrophilic components by high-performance liquid chromatography with ultraviolet detection

  • Ryusei Oshima
  • Akira KotaniEmail author
  • Minpei Kuroda
  • Kazuhiro Yamamoto
  • Yoshihiro Mimaki
  • Hideki Hakamata
Original Paper
  • 276 Downloads

Abstract

High-performance liquid chromatography with ultraviolet detection (HPLC–UV) using 20 mM phosphate mobile phase and an octadecylsilyl column (Triart C18, 150 × 3.0 mm i.d., 3 μm) has been developed for the analysis of hydrophilic compounds in the water extract of Schisandrae Fructus samples. The present HPLC–UV method permits the accurate and precise determination of malic, citric, and protocatechuic acids in the Japanese Pharmacopoeia (JP) Schisandrae Fructus, Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus. The JP Schisandrae Fructus studied contains 27.98 mg/g malic, 107.08 mg/g citric, and 0.42 mg/g protocatechuic acids, with a relative standard deviation (RSD) of repeatability of <0.9% (n = 6). The content of malic acids in Schisandrae Chinensis Fructus is approximately ten times that in Schisandrae Sphenantherae Fructus. To examine whether the HPLC–UV method is applicable to the fingerprint-based discrimination of Schisandrae Fructus samples obtained from Chinese markets, principal component analysis (PCA) was performed using the determined contents of organic acids and the ratio of six characteristic unknown peaks derived from hydrophilic components to internal standard peak areas. On the score plots, Schisandrae Chinensis Fructus and Schisandrae Sphenantherae Fructus samples are clearly discriminated. Therefore, the HPLC–UV method for the analysis of hydrophilic components coupled with PCA has been shown to be practical and useful in the quality control of Schisandrae Fructus.

Keywords

Organic acid Schisandrae Chinensis Fructus Schisandrae Sphenantherae Fructus Discrimination Principal component analysis 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

11418_2017_1158_MOESM1_ESM.pdf (146 kb)
Supplementary material 1 (PDF 145 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Analytical Chemistry, School of PharmacyTokyo University of Pharmacy and Life SciencesTokyoJapan
  2. 2.Department of Medicinal Pharmacognosy, School of PharmacyTokyo University of Pharmacy and Life SciencesTokyoJapan

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