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Journal of Natural Medicines

, Volume 72, Issue 1, pp 342–346 | Cite as

AU-1 from Agavaceae plants downregulates the expression of glycolytic enzyme phosphoglycerate mutase

  • Tomofumi FujinoEmail author
  • Akihito Yokosuka
  • Harutaka Ichikawa
  • Toshiyuki Oshima
  • Yoshihiro Mimaki
  • Makio Hayakawa
Note
  • 201 Downloads

Abstract

The spirostanol saponin AU-1 from Agavaceae plants stimulates the expression of the glycolytic enzyme phosphoglycerate mutase (PGAM) in ACHN cells. We hypothesized that this may arise from the downregulation of the NAD+-dependent deacetylase SIRT1. In this article, we showed that, unlike in renal adenocarcinoma cells, AU-1 does not affect the expression of SIRT1 in the normal renal cell-derived cell line HK-2. Consistent with the lack of a downregulation of SIRT1, AU-1 did not upregulate, but rather decreased PGAM expression. Moreover, AU-1 inhibited the increase in PGAM levels that results from the knock-down of SIRT1. Our results suggest that AU-1 may prevent carcinogenesis caused by increased cellular PGAM.

Keywords

Agave utahensis Phosphoglycerate mutase SIRT1 

Notes

Acknowledgements

We thank Ken Ando for their helpful advice and discussions. This work was supported in part by a grant from the Japan Private School Promotion Foundation.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Tomofumi Fujino
    • 1
    Email author
  • Akihito Yokosuka
    • 2
  • Harutaka Ichikawa
    • 1
  • Toshiyuki Oshima
    • 1
  • Yoshihiro Mimaki
    • 2
  • Makio Hayakawa
    • 1
  1. 1.Department of Hygiene and Health Sciences, School of PharamacyTokyo University of Pharmacy and Life SciencesHachiojiJapan
  2. 2.Department of Medicinal Pharmacognosy, School of PharamacyTokyo University of Pharmacy and Life SciencesHachiojiJapan

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