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Journal of Natural Medicines

, Volume 72, Issue 1, pp 267–273 | Cite as

Botanical origin and chemical constituents of commercial Saposhnikoviae radix and its related crude drugs available in Shaanxi and the surrounding regions

  • Takuro MaruyamaEmail author
  • Masami Ezaki
  • Mao Shiba
  • Hiroki Yamaji
  • Taichi Yoshitomi
  • Noriaki Kawano
  • Shu Zhu
  • Xiao Cheng
  • Tsuguo Yokokura
  • Yutaka Yamamoto
  • Hiroyuki Fuchino
  • Hang Sun
  • Katsuko Komatsu
  • Nobuo Kawahara
Original Paper
  • 226 Downloads

Abstract

Saposhnikoviae radix (SR) is described in the Japanese Pharmacopoeia as a crude drug derived from the root of Saposhnikovia divaricata Schischkin (Umbelliferae). According to Flora of China, the root of Peucedanum ledebourielloides K. F. Fu is used as a regional substitute for SR. Therefore, we surveyed the botanical origin of the drug used in China, especially Shaanxi and the surrounding regions, through nucleotide sequence analysis of the internal transcribed spacer region of rDNA. As a result, several samples from Shaanxi (陝西) and Shanxi (山西) provinces were identified as Peucedanum ledebourielloides. To prevent this substitute from being distributed as genuine SR, we developed a thin-layer chromatography analysis condition to enable a specific compound of this species to be easily detected. The specific compound was identified as xanthalin, based on 1D- and 2D-NMR and high-resolution mass spectrometry data. The established TLC conditions were as follows—extraction solvent, n-hexane; applied volume, 5 µL; chromatographic support, silica gel; developing solvent, n-hexane:ethyl acetate:acetic acid (20:10:1); developing length, 7 cm; detection, UV (365 nm); R f value, 0.4 (blue fluorescence; xanthalin).

Keywords

Saposhnikoviae radix Shaanxi province Peucedanum ledebourielloides TLC Xanthalin 

Notes

Acknowledgements

Part of this study was supported by a research grant from the Japan Agency for Medical Research and Development.

Supplementary material

11418_2017_1149_MOESM1_ESM.tiff (1.2 mb)
Fig. S1 LC–MS chromatograms for S. divaricata and P. ledebourielloides root (TIFF 1253 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  • Takuro Maruyama
    • 1
    Email author
  • Masami Ezaki
    • 2
  • Mao Shiba
    • 3
  • Hiroki Yamaji
    • 3
  • Taichi Yoshitomi
    • 1
  • Noriaki Kawano
    • 2
  • Shu Zhu
    • 4
  • Xiao Cheng
    • 5
  • Tsuguo Yokokura
    • 6
  • Yutaka Yamamoto
    • 7
  • Hiroyuki Fuchino
    • 2
  • Hang Sun
    • 5
  • Katsuko Komatsu
    • 4
  • Nobuo Kawahara
    • 2
  1. 1.Division of Pharmacognosy, Phytochemistry and NarcoticsNational Institute of Health SciencesKawasaki, KanagawaJapan
  2. 2.Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and NutritionTsukubaJapan
  3. 3.Botanical Raw Materials Research LaboratoriesTsumura & Co.Ami-machiJapan
  4. 4.Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural MedicineUniversity of ToyamaToyamaJapan
  5. 5.Kunming Institute of Botany, Chinese Academy of ScienceKunmingChina
  6. 6.Nippon Funmatsu Yakuhin Co., Ltd.OsakaJapan
  7. 7.Tochimoto Tenkaido Co., Ltd.TambaJapan

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