The binding properties of recombinant antibody fragments, such as affinity and specificity, determine their usefulness for therapeutic and analytical applications. Anti-paclitaxel single-chain variable fragment clone 1C2 (anti-PT scFv1C2) was expressed using Escherichia coli cell and Bombyx mori larvae expression systems. The binding characteristics of the scFvs were evaluated using indirect competitive ELISA. The linear range of binding between anti-PT scFv1C2 and paclitaxel was significantly different between the anti-PT scFv1C2s derived from E. coli (1.056–5.700 μg/ml) and B. mori (7.813–1000 ng/ml), which indicated that different expression systems resulted in different sensitivities for paclitaxel determination. In addition, the binding specificity of anti-PT scFv1C2 varied between expression systems. This finding implied that the expression system significantly affects the binding properties of recombinant antibodies, especially antibodies against low-molecular-weight targets.
Single-chain variable fragment Expression systems Specificity Analytical performance
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We acknowledge financial support from the Japan Society for the Promotion of Science (No. 22501046 and No. 19590119) and from the Takeda Science Foundation. We also appreciate the technical support from the Research Support Center, Graduate School of Medical Sciences, Kyushu University, Japan.
Compliance with ethical standards
Conflict of interest
The authors have declared no conflict of interest.
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