Journal of Natural Medicines

, Volume 72, Issue 1, pp 290–297 | Cite as

Labdane-type diterpenoids from Vitex limonifolia and their antivirus activities

  • Ninh Khac Ban
  • Nguyen Thi Kim Thoa
  • Tran My Linh
  • Do Thi Trang
  • Phan Van Kiem
  • Nguyen Xuan Nhiem
  • Bui Huu Tai
  • Chau Van Minh
  • Jae-Hyoung Song
  • Hyun-Jeong Ko
  • Seung Hyun KimEmail author


Phytochemical investigation of the methanol extract of Vitex limonifolia leaves led to the isolation of three new labdane-type diterpenoids, vitexlimolides A–C (1–3) and eight known compounds, 5,4′-dihydroxy-3,7-dimethoxyflavone (4), vitecetin (5), 5,4′-dihydroxy-7,3′-dimethoxyflavone (6), verrucosin (7), 2α, 3α-dihydroxy-urs-12-en-28-oic acid (8), euscaphlic acid (9), 18,19-seco, 2α, 3α-dihydroxy-19-oxo-urs-11,13(18)-dien-28-oic acid (10), and maslinic acid (11). Their chemical structures were elucidated by physical and chemical methods. All compounds were evaluated for antiviral activities against CVB3, HRV1B, and EV71 viruses. As a result, compounds 4 and 6 showed potent antiviral activity against CVB3 infection with IC50 values of 0.12 ± 0.06 and 1.86 ± 0.18 (µM), respectively.

Graphical abstract


Coxsackievirus B3 Enterovirus 71 Human rhinovirus Labdane Vitex limonifolia 



This research is funded by the Vietnam National Foundation for Science and Technology Development (NAFOSTED) under Grant Number 104.01-2014.02 and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2011-0025129).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

11418_2017_1125_MOESM1_ESM.doc (1.2 mb)
Supplementary material 1 (DOC 1232 kb)


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  • Ninh Khac Ban
    • 1
  • Nguyen Thi Kim Thoa
    • 3
  • Tran My Linh
    • 1
  • Do Thi Trang
    • 1
  • Phan Van Kiem
    • 1
  • Nguyen Xuan Nhiem
    • 1
  • Bui Huu Tai
    • 1
  • Chau Van Minh
    • 1
  • Jae-Hyoung Song
    • 4
  • Hyun-Jeong Ko
    • 4
  • Seung Hyun Kim
    • 2
    Email author
  1. 1.Institute of Marine BiochemistryVietnam Academy of Science and Technology (VAST)HanoiVietnam
  2. 2.College of Pharmacy, Yonsei Institute of Pharmaceutical ScienceYonsei UniversityIncheonRepublic of Korea
  3. 3.Faculty of Basic SciencesHanoi University of Mining and GeologyHanoiVietnam
  4. 4.College of PharmacyKangwon National UniversityChuncheonRepublic of Korea

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