Journal of Natural Medicines

, Volume 72, Issue 1, pp 127–135 | Cite as

Whitening and anti-wrinkle activities of ferulic acid isolated from Tetragonia tetragonioides in B16F10 melanoma and CCD-986sk fibroblast cells

  • Hye-Jin Park
  • Jun-Hyo Cho
  • Shin-Hyub Hong
  • Dong-Hee Kim
  • Hee-Young Jung
  • In-Kyu Kang
  • Young-Je ChoEmail author
Original Paper


Ferulic acid isolated from Tetragonia tetragonioides was tested for its whitening effect on the B16F10 mouse melanoma cell line and its anti-wrinkle activity on the CCD-986sk human dermal fibroblast cell line. Ferulic acid, one of the primary phenolic compounds that can be isolated from T. tetragonioides, has been reported to show potential as a functional food, for its whitening effect and anti-wrinkle activity. To measure its whitening and anti-wrinkle activities, cells were treated with ferulic acid isolated from T. tetragonioides at concentrations between 5 and 20 μM. Ferulic acid showed no cytotoxicity at concentrations up to 20 μM. Ferulic acid inhibited melanin synthesis, tyrosinase expression, and microphthalmia transcription factor expression in B16F10 cells stimulated with α-melanocyte stimulating hormone. Ferulic acid induced procollagen synthesis, hyaluronic acid synthesis, tissue inhibitor of metalloproteinase synthesis, and inhibited matrix metalloproteinase (MMP)-1 and MMP-9 expression in CCD-986sk cells stimulated with UV-B. On the basis of these results, we conclude that ferulic acid isolated from T. tetragonioides shows potential for use as a functional food, with whitening and anti-wrinkle activities.


Tetragonia tetragonioides Ferulic acid B16F10 mouse melanoma cells CCD-986sk human dermal fibroblasts Whitening activity Anti-wrinkle activity 


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  • Hye-Jin Park
    • 1
  • Jun-Hyo Cho
    • 1
  • Shin-Hyub Hong
    • 1
  • Dong-Hee Kim
    • 2
  • Hee-Young Jung
    • 3
  • In-Kyu Kang
    • 4
  • Young-Je Cho
    • 1
    Email author
  1. 1.School of Food Science and Biotechnology/Institute of Agricultural Science and TechnologyKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Applide Product Development TeamNational Development Institute of Korean MedicineGyeongsanRepublic of Korea
  3. 3.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  4. 4.Department of Horticultural ScienceKyungpook National UniversityDaeguRepublic of Korea

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