Journal of Natural Medicines

, Volume 72, Issue 1, pp 106–117 | Cite as

Hepatoprotective and cytotoxic activities of Anvillea garcinii and isolation of four new secondary metabolites

  • Shagufta PerveenEmail author
  • Areej Mohammad Al-Taweel
  • Hasan Soliman Yusufoglu
  • Ghada Ahmed Fawzy
  • Ahmed Foudah
  • Maged Saad Abdel-Kader
Original Paper


Anvillea garcinii is a medicinal plant traditionally used for the treatment of dysentery, gastrointestinal troubles, hepatitis, lung disease, colds, digestive problems and pulmonary affections and in liver diseases. Four new sesquiterpene lactones, garcinamines A–D, along with seven known compounds, were isolated from the leaves of A. garcinii. This is the first report of the isolation of amino acid analogues of parthenolide-type sesquiterpene lactones from the family Asteraceae. Total ethanol extract of leaves as well as the chloroform and n-butanol fractions were tested for their hepatoprotective effect using the carbon tetrachloride liver toxicity model. The chloroform fraction, at a dose of 400 mg/kg, demonstrated a significant hepatoprotective effect comparable to silymarin in all serum and tissue parameters. The cytotoxicity of all extracts and compounds were evaluated against five human cancer cell lines: MCF-7, HCT-116, HepG2, Hela and A-549. The results indicated that the chloroform and n-butanol fractions and compounds 3, 4, 7 and 8 displayed significant cytotoxic activity against these cell lines.


Anvillea garcinii Sesquiterpene lactones Hepatoprotective activity Cytotoxicity 



This research project was supported by a grant from the “Research Center of the Female Scientific and Medical Colleges”, Deanship of Scientific Research, King Saud University.

Compliance with ethical standards

Conflict of interest

The authors declare they have no conflicts of interest.

Supplementary material

11418_2017_1118_MOESM1_ESM.pdf (1.3 mb)
Supplementary material 1 (PDF 1293 kb)


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  • Shagufta Perveen
    • 1
    Email author
  • Areej Mohammad Al-Taweel
    • 1
  • Hasan Soliman Yusufoglu
    • 2
  • Ghada Ahmed Fawzy
    • 1
    • 3
  • Ahmed Foudah
    • 2
  • Maged Saad Abdel-Kader
    • 2
    • 4
  1. 1.Department of Pharmacognosy, College of PharmacyKing Saud UniversityRiyadhKingdom of Saudi Arabia
  2. 2.Department of Pharmacognosy, College of PharmacyPrince Sattam Bin Abdulaziz UniversityAl-KharjSaudi Arabia
  3. 3.Department of Pharmacognosy, Faculty of PharmacyCairo UniversityCairoEgypt
  4. 4.Department of Pharmacognosy, Faculty of PharmacyAlexandria UniversityAlexandriaEgypt

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