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Journal of Natural Medicines

, Volume 72, Issue 1, pp 80–95 | Cite as

Comparison of chemical constituents among licorice, roasted licorice, and roasted licorice with honey

  • Misato Ota
  • Feng Xu
  • Yao-Li Li
  • Ming-Ying Shang
  • Toshiaki Makino
  • Shao-Qing CaiEmail author
Original Paper

Abstract

Licorice (root and rhizome of Glycyrrhiza uralensis Fisch.) is sometimes used as crude drug after processing. In this report, we prepared roasted licorice with or without honey using 3 lots of crude drug samples derived from wild G. uralensis, and analyzed the constituents in unprocessed, roasted, and honey-roasted licorice samples by high performance liquid chromatography–electrospray ionization-ion trap-time of flight mass spectrometry (HPLC–ESI-IT-TOF-MSn) with principal component analysis. We found that the areas of 41 peaks were noticeably changed by processing. Among them, the areas of 12 peaks, viz. isoliquiritin, isoliquiritigenin, glucoisoliquiritin, 6″-O-acetylisoliquiritin, 6″-O-acetylisoliquiritin apioside, glycyrrhetinic acid 3-O-glucuronide, 5 kinds of sugar-derivatives and one compound whose molecular weight was 386 Da were increased by roasting in all 3 lots, and those peak areas were increased by higher heating temperatures. Among the increased peaks, 3 kinds of sugar-derivatives had larger areas, and 6″-O-acetylisoliquiritin had lower areas than those in honey-roasted licorice. Those sugar-derivatives were the only characteristics differing between honey-roasted licorice and roasted licorice. Meanwhile, the areas of 9 peaks, four of them identified as 6″-O-acetylliquiritin, 6″-O-acetylliquiritin apioside, formononetin and gancaonin l, were decreased by roasting in all 3 lots, but there were no differences between roasted licorice with or without honey. Those compounds whose amounts were changed by processing could be used as markers for the quality control of roasted and honey-roasted licorice.

Keywords

Licorice Processing Roasting Honey HPLC–ESI-IT-TOF-MSn 

Supplementary material

11418_2017_1115_MOESM1_ESM.docx (34 kb)
Supplementary material 1 (DOCX 33 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  • Misato Ota
    • 1
    • 2
  • Feng Xu
    • 1
  • Yao-Li Li
    • 1
  • Ming-Ying Shang
    • 1
  • Toshiaki Makino
    • 2
  • Shao-Qing Cai
    • 1
    Email author
  1. 1.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical SciencesPeking University Health Science CenterBeijingChina
  2. 2.Department of Pharmacognosy, Graduate School of Pharmaceutical SciencesNagoya City UniversityNagoyaJapan

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