Advertisement

Journal of Natural Medicines

, Volume 70, Issue 3, pp 610–619 | Cite as

Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice

  • Hongxia Chen
  • Opeyemi J. Olatunji
  • Yifeng Zhou
Original Paper

Abstract

The evaluation of the antioxidant, anti-secretory and anti-inflammatory potentials of the ethyl acetate fraction (ELC) from the root bark of Lycium chinense against ethanol-induced gastric ulcer in mice and the possible mechanisms underlying this action was performed. The results indicated that oral administration of ELC (50, 100, 200 and 400 mg/kg) before ethanol-induced ulcer increased the gastric mucus content, restored the superoxide dismutase and glutathione levels, reduced malondialdehyde levels and inhibited the activity of myeloperoxidase. Furthermore, ELC displayed its anti-secretory activity by decreasing the gastric juice and increased the gastric pH and reduced pro-inflammatory markers and caspase-3 tissue levels. These results suggest that L. chinense displays gastroprotective properties as a result of its antioxidant, anti-inflammatory, anti-secretory and anti-apoptotic effects.

Keywords

Lycium chinense Cortex Lycii Antioxidant Ethanol Anti-inflammatory 

Notes

Acknowledgments

Thanks go to the Public Science and Technology Research Funds Projects of Ocean (no. 201505023) and the Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm (no. BM2014047) for the financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest related to this article.

References

  1. 1.
    Ye Z, Huang Q, Ni HX, Wang D (2008) Cortex Lycii Radicis extracts improve insulin resistance and lipid metabolism in obese-diabetic rats. Phytother Res 22:1665–1670CrossRefPubMedGoogle Scholar
  2. 2.
    Yao X, Peng Y, Xu LJ, Li L, Wu QL, Xiao PG (2011) Phytochemical and biological studies of Lycium medicinal plants. Chem Biodivers 8:976–1010CrossRefPubMedGoogle Scholar
  3. 3.
    Wang L, Waltenberger B, Pferschy-Wenzig EM, Blunder M, Liu X, Malainer C, Blazevic T, Schwaiger S, Rollinger JM, Heiss EH, Schuster D, Kopp B, Bauer R, Stuppner H, Dirsch VM, Atanasov AG (2014) Natural product agonists of peroxisome proliferator-activated receptor gamma (PPARγ): a review. Biochem Pharmacol 92:73–89CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Zhang JX, Guan SH, Feng RH, Wang Y, Wu ZY, Zhang YB, Chen XH, Bi KS, Guo DA (2013) Neolignanamides, lignanamides, and other phenolic compounds from the root bark of Lycium chinense. J Nat Prod 76:51–58CrossRefPubMedGoogle Scholar
  5. 5.
    Mocan A, Vlase L, Vodnar DC, Bischin C, Hanganu D, Gheldiu AM, Oprean R, Silaghi-Dumitrescu R, Crişan G (2014) Polyphenolic content, antioxidant and antimicrobial activities of Lycium barbarum L. and Lycium chinense Mill. leaves. Molecules 19:10056–10073CrossRefPubMedGoogle Scholar
  6. 6.
    Potterat O (2010) Goji (Lycium barbarum and L. chinense): phytochemistry, pharmacology and safety in the perspective of traditional uses and recent popularity. Planta Med 76:7–19CrossRefPubMedGoogle Scholar
  7. 7.
    Jung K, Chin YW, Kim YC, Kim J (2005) Potentially hepatoprotective glycolipid constituents of Lycium chinense fruits. Arch Pharm Res 28:1381–1385CrossRefPubMedGoogle Scholar
  8. 8.
    Chin YW, Lim SW, Kim SH, Shin DY, Suh YG, Kim YB, Kim YC, Kim J (2003) Hepatoprotective pyrrole derivatives of Lycium chinense fruits. Bioorg Biomed Chem Lett 13:79–81CrossRefGoogle Scholar
  9. 9.
    Lee DG, Jung HJ, Woo ER (2005) Antimicrobial property of (+)-lyoniresinol-3alpha-O-beta-d-glucopyranoside isolated from the root bark of Lycium chinense Miller against human pathogenic microorganisms. Arch Pharm Res 28:1031–1036CrossRefPubMedGoogle Scholar
  10. 10.
    Gao D, Li Q, Liu Z, Li Y, Liu Z, Fan Y, Li K, Han Z, Li J (2007) Hypoglycemic effects and mechanisms of action of Cortex Lycii Radicis on alloxan-induced diabetic mice. Yakugaku Zasshi 127:1715–1721CrossRefPubMedGoogle Scholar
  11. 11.
    Behrman SW (2005) Management of complicated peptic ulcer disease. Arch Surg 140:201–208CrossRefPubMedGoogle Scholar
  12. 12.
    Caldas GF, do Amaral Costa IM, da Silva JB, da Nóbrega RF, Rodrigues FF, da Costa JG, Wanderley AG (2011) Antiulcerogenic activity of the essential oil of Hyptis martiusii Benth. (Lamiaceae). J Ethnopharmacol 137:886–892CrossRefPubMedGoogle Scholar
  13. 13.
    Klein LC Jr, Gandolfi RB, Santin JR, Lemos M, Cechinel Filho V, de Andrade SF (2010) Antiulcerogenic activity of extract, fractions, and some compounds obtained from Polygala cyparissias St. Hillaire & Moquin (Polygalaceae). Naunyn Schmiedebergs Arch Pharmacol 381:121–126CrossRefPubMedGoogle Scholar
  14. 14.
    da Silva LM, Allemand A, Mendes DA, Dos Santos AC, André E, de Souza LM, Cipriani TR, Dartora N, Marques MC, Baggio CH, Werner MF (2013) Ethanolic extract of roots from Arctium lappa L. accelerates the healing of acetic acid-induced gastric ulcer in rats: involvement of the antioxidant system. Food Chem Toxicol 51:179–187CrossRefPubMedGoogle Scholar
  15. 15.
    DeVault KR, Talley NJ (2009) Insights into the future of gastric acid suppression. Nat Rev Gastroenterol Hepatol 6:524–532CrossRefPubMedGoogle Scholar
  16. 16.
    Kangwan N, Park JM, Kim EH, Hahm KB (2014) Quality of healing of gastric ulcers: natural products beyond acid suppression. World J Gastrointest Pathophysiol 5:40–47CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Marotta F, Tajiri H, Safran P, Fesce E, Ideo G (1999) Ethanol-related gastric mucosal damage: evidence of a free radical-mediated mechanism and beneficial effect of oral supplementation with bionormalizer, a novel natural antioxidant. Digestion 60:538–543CrossRefPubMedGoogle Scholar
  18. 18.
    Suzuki H, Matsuzaki J, Hibi T (2011) Ghrelin and oxidative stress in gastrointestinal tract. J Clin Biochem Nutr 48:122–125CrossRefPubMedGoogle Scholar
  19. 19.
    Tuorkey M, Karolin K (2009) Anti-ulcer activity of curcumin on experimental gastric ulcer in rats and its effect on oxidative stress/antioxidant, IL-6 and enzyme activities. Biomed Environ Sci 22:488–495CrossRefPubMedGoogle Scholar
  20. 20.
    Chattopadhyay I, Bandyopadhyay U, Biswas K, Maity P, Banerjee RK (2006) Indomethacin inactivates gastric peroxidase to induce reactive-oxygen-mediated gastric mucosal injury and curcumin protects it by preventing peroxidase inactivation and scavenging reactive oxygen. Free Radic Biol Med 40:1397–1408CrossRefPubMedGoogle Scholar
  21. 21.
    Olatunji OJ, Chen H, Zhou Y (2015) Anti-ulcerogenic properties of Lycium chinense Mill extracts against ethanol-induced acute gastric lesion in animal models and its active constituents. Molecules 20:22553–22564CrossRefPubMedGoogle Scholar
  22. 22.
    Shay H, Komarov SA, Fels SS, Meranze D, Gruenstein M, Siplet H (1945) A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 5:43–61Google Scholar
  23. 23.
    Al-Sayed E, El-Naga RN (2015) Protective role of ellagitannins from Eucalyptus citriodora against ethanol-induced gastric ulcer in rats: impact on oxidative stress, inflammation and calcitonin-gene related peptide. Phytomedicine 22:5–15CrossRefPubMedGoogle Scholar
  24. 24.
    Laine L, Weinstein WM (1988) Histology of alcoholic hemorrhagic “gastritis”: a prospective evaluation. Gastroenterology 94:1254–1262CrossRefPubMedGoogle Scholar
  25. 25.
    Yang WJ, Liu C, Gu ZY, Zhang XY, Cheng B, Mao Y, Xue GP (2014) Protective effects of acacetin isolated from Ziziphora clinopodioides Lam. (Xintahua) on neonatal rat cardiomyocytes. Chin Med 9:28CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Bergman M, Varshavsky L, Gottlieb HE, Grossman S (2001) The antioxidant activity of aqueous spinach extract: chemical identification of active fractions. Phytochemistry 58:143–152CrossRefPubMedGoogle Scholar
  27. 27.
    Miyazawa M, Hisama M (2003) Antimutagenic activity of flavonoids from Chrysanthemum morifolium. Biosci Biotechnol Biochem 67:2091–2099CrossRefPubMedGoogle Scholar
  28. 28.
    Liu YB, Cheng XR, Qin JJ, Yan SK, Jin HZ, Zhang WD (2011) Chemical constituents of Toona ciliata var. pubescens. Chin J Nat Med 9:115–119Google Scholar
  29. 29.
    Zhao XH, Chen DH, Si JY, Pan RL, Shen LG (2002) Studies on the phenolic acid constituents from Chinese medicine “sheng-ma”, rhizome of Cimicifuga foetida L. Acta Pharm Sin 37:535–538Google Scholar
  30. 30.
    Lin LC, Pai YF, Tsai TH (2015) Isolation of luteolin and luteolin-7-O-glucoside from Dendranthema morifolium Ramat Tzvel and their pharmacokinetics in rats. J Agric Food Chem 63:7700–7706CrossRefPubMedGoogle Scholar
  31. 31.
    Pan S, Wang X, Duan W, Yu Z, Zhang L, Liu W (2014) Preparative isolation and purification of flavonoids from Cuscuta chinensis Lam. by high-speed countercurrent chromatography. J Liq Chrom Relat Tech 37:2162–2171CrossRefGoogle Scholar
  32. 32.
    Lee DG, Park Y, Kim MR, Jung HJ, Seu YB, Hahm KS, Woo ER (2004) Anti-fungal effects of phenolic amides isolated from the root bark of Lycium chinense. Biotechnol Lett 26:1125–1130CrossRefPubMedGoogle Scholar
  33. 33.
    Zhao XM, Ye XQ, Zhu DY (2008) A novel compound isolated from the peels of Citrus changshan-huyou. Acta Pharm Sin 43:1208–1210Google Scholar
  34. 34.
    Fernández S, Wasowski C, Paladini AC, Marder M (2004) Sedative and sleep-enhancing properties of linarin, a flavonoid-isolated from Valeriana officinalis. Pharmacol Biochem Behav 77:399–404CrossRefPubMedGoogle Scholar
  35. 35.
    Sidahmed HM, Hashim NM, Amir J, Abdulla MA, Hadi AH, Abdelwahab SI, Taha MM, Hassandarvish P, Teh X, Loke MF, Vadivelu J, Rahmani M (2013) Pyranocycloartobiloxanthone A, a novel gastroprotective compound from Artocarpus obtusus Jarret, against ethanol-induced acute gastric ulcer in vivo. Phytomedicine 20:834–843CrossRefPubMedGoogle Scholar
  36. 36.
    Wallace JL (2008) Prostaglandins, NSAIDs, and gastric mucosal protection: why doesn’t the stomach digest itself? Physiol Rev 88:1547–1565CrossRefPubMedGoogle Scholar
  37. 37.
    Sairam KC, Rao CV, Babu MD, Kumar KV, Agrawal VK, Goel RK (2002) Antiulcerogenic effect of methanolic extract of Emblica officinalis: an experimental study. J Ethnopharmacol 82:1–9CrossRefPubMedGoogle Scholar
  38. 38.
    Wassmann S, Stumpf M, Strehlow K, Schmid A, Schieffer B, Böhm M, Nickenig G (2004) Interleukin-6 induces oxidative stress and endothelial dysfunction by overexpression of the angiotensin II type 1 receptor. Circ Res 94:534–541CrossRefPubMedGoogle Scholar
  39. 39.
    Bonamin F, Moraes TM, Dos Santos RC, Kushima H, Faria FM, Silva MA, Junior IV, Nogueira L, Bauab TM, Souza Brito ARM, da Rocha LRM, Hiruma-Lima CA (2014) The effect of a minor constituent of essential oil from Citrus aurantium: the role of β-myrcene in preventing peptic ulcer disease. Chem Biol Interact 212:11–19CrossRefPubMedGoogle Scholar
  40. 40.
    Moezi L, Janahmadi Z, Amirghofran Z, Nekooeian AA, Dehpour AR (2014) The increased gastroprotective effect of pioglitazone in cholestatic rats: role of nitric oxide and tumour necrosis factor alpha. Int J Exp Pathol 95:78–85CrossRefPubMedPubMedCentralGoogle Scholar
  41. 41.
    Warzecha Z, Ceranowicz P, Dembinski M, Cieszkowski J, Ginter G, Ptak-Belowska A, Dembinski A (2014) Involvement of cyclooxygenase-1 and cyclooxygenase-2 activity in the therapeutic effect of ghrelin in the course of ethanol-induced gastric ulcers in rats. J Physiol Pharmacol 65:95–106PubMedGoogle Scholar
  42. 42.
    Luo XJ, Liu B, Dai Z, Li TB, Li NS, Zhang XJ, Yang ZC, Li YJ, Peng J (2013) Expression of apoptosis-associated microRNAs in ethanol-induced acute gastric mucosal injury via JNK pathway. Alcohol 47:481–493CrossRefPubMedGoogle Scholar
  43. 43.
    Arab HH, Salama SA, Omar HA, Arafa el-SA, Maghrabi IA (2015) Diosmin protects against ethanol-induced gastric injury in rats: novel anti-ulcer actions. PLoS One 10:e0122417CrossRefPubMedPubMedCentralGoogle Scholar
  44. 44.
    Yu J, Peng H, Lin Y, Yi S (2013) Effect of moxibustion treatment on cell apoptosis and expressions of heat shock protein and second mitochondrial activator of caspase in acute gastric mucosal lesion of rats. J Tradit Chin Med 33:258–261CrossRefPubMedGoogle Scholar
  45. 45.
    Nzowa LK, Barboni L, Teponno RB, Ricciutelli M, Lupidi G, Quassinti L, Bramucci M, Tapondjou LA (2010) Rheediinosides A and B, two antiproliferative and antioxidant triterpene saponins from Entada rheedii. Phytochemistry 71:254–261CrossRefPubMedGoogle Scholar
  46. 46.
    Milner JA (1994) Reducing the risk of cancer. In: Goldberg I (ed) Functional foods: designer foods, pharmafoods, nutraceuticals. Chapman & Hall, New York, NY, pp 39–70CrossRefGoogle Scholar
  47. 47.
    Duthie GG, Brown KM (1994) Reducing the risk of cardiovascular disease. In: Goldberg I (ed) Functional foods: designer foods, pharmafoods, nutraceuticals. Chapman & Hall, New York, NY, pp 19–38CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2016

Authors and Affiliations

  • Hongxia Chen
    • 1
    • 2
  • Opeyemi J. Olatunji
    • 2
  • Yifeng Zhou
    • 1
    • 3
  1. 1.Institute of Botany, Jiangsu Province and Chinese Academy of SciencesNanjing Botanical Garden Mem. Sun Yat-senNanjingChina
  2. 2.School of PharmacyJiangsu UniversityZhenjiangChina
  3. 3.Dongtai Institute of Tidal FlatNanjing Branch of Chinese Academy of SciencesDongtaiChina

Personalised recommendations