Journal of Natural Medicines

, Volume 70, Issue 2, pp 271–275 | Cite as

Composition of the endophytic filamentous fungi associated with Cinchona ledgeriana seeds and production of Cinchona alkaloids

  • Shoji MaeharaEmail author
  • Andria Agusta
  • Chinami Kitamura
  • Kazuyoshi Ohashi
  • Hirotaka Shibuya


Four kinds of endophytic filamentous fungi (code names: CLS-1, CLS-2, CLS-3, and CLS-4) associated with the seeds of Cinchona ledgeriana (Rubiaceae) from West Java, Indonesia, were isolated. All of the isolates were classified into Diaporthe spp. based on phylogenetic analysis of the nucleotide sequences of the internal transcribed spacers (ITS1 and ITS2) including the 5.8S ribosomal DNA region. All four of these endophytic fungi produce Cinchona alkaloids, mainly quinine and quinidine, in synthetic liquid medium.


Endophytic filamentous fungus Cinchona alkaloid Cinchona ledgeriana Quinine Quinidine 


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2015

Authors and Affiliations

  • Shoji Maehara
    • 1
    Email author
  • Andria Agusta
    • 2
  • Chinami Kitamura
    • 1
  • Kazuyoshi Ohashi
    • 1
  • Hirotaka Shibuya
    • 1
  1. 1.Faculty of Pharmacy and Pharmaceutical SciencesFukuyama UniversityFukuyamaJapan
  2. 2.Research Center for BiologyIndonesian Institute of SciencesCibinong, BogorIndonesia

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