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Journal of Natural Medicines

, Volume 69, Issue 3, pp 427–431 | Cite as

Melanin biosynthesis inhibitory activity of a compound isolated from young green barley (Hordeum vulgare L.) in B16 melanoma cells

  • Tian Xiao Meng
  • Nobuto Irino
  • Ryuichiro KondoEmail author
Note

Abstract

In the course to find compounds that inhibit melanin biosynthesis (i.e., whitening agents), we evaluated the effects of the methanol-soluble fraction (i.e., the water-soluble portion of methanol extracts-CHP20P-MeOH eluted fraction) from young green barley leaves on melanin production in B16 melanoma cells. Activity-guided fractionation led to an isolate called tricin (compound 1) as an inhibitory compound of melanin production in B16 melanoma cells. Furthermore, tricin analogs such as tricetin, tricetin trimethyl ether, luteolin, and apigenin were used for analyzing the structure–activity relationships (SAR) of 5,7-dihydroxyflavones studies. Tricin demonstrated stronger inhibitory activity compared to three other compounds. The results suggest that a hydroxyl group at the C-4′ position and methoxy groups at the C-3′,5′ positions of the tricin skeleton may have important roles in this inhibitory activity in B16 melanoma cells. Our results suggest that tricin inhibits melanin biosynthesis with higher efficacy than arbutin, and it could be used as a whitening agent.

Keywords

Young green barley Melanin inhibition activity Tricin Whitening agent 

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2015

Authors and Affiliations

  • Tian Xiao Meng
    • 1
  • Nobuto Irino
    • 2
  • Ryuichiro Kondo
    • 1
    Email author
  1. 1.Faculty of AgricultureKyushu UniversityFukuokaJapan
  2. 2.Asahi Ryokuken Co., Ltd.FukuokaJapan

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